Xtt substrate

XTT cell viability assays were performed by first seeding 4000 LM2-4 cells into a 96-well tissue culture plate. After cells attached overnight the indicated concentrations of Reversine (Selleckchem) or vehicle (DMSO) were then added to the wells in 100 μL phenol-free complete DMEM medium. After 48 h, XTT substrate (Thermo Fisher Scientific) was added to the wells and incubated for 2 h before reading the A450 nm on a plate reader.

XTT cell viability assays were performed by first seeding cells into a 96-well tissue culture plate at the following density per well: LM2-4 (4,000), BT549 (2,000), HCC38 (4,000), or MDA-MB-436 (7,500). After cells attached overnight the indicated concentrations of Olaparib (Lynparza) (Selleckchem, Houston, TX, USA) or vehicle alone (DMSO) were then added to the wells in 100 μL phenol-free complete DMEM medium. After 48 h, XTT substrate (Thermo Fisher Scientific) was added to the wells and incubated for 2 h before reading the A450 nm on a plate reader. Cell viability for each of the indicated treatments was expressed as a percent of the vehicle control wells.