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F8192 500ml

Manufactured by Thermo Fisher Scientific
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The F8192-500ML is a laboratory equipment product by Thermo Fisher Scientific. It is a container with a capacity of 500 milliliters.

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Lab products found in correlation

Tlrl peklps, by InvivoGen (2 mentions) P1585 1mg, by Merck Group (2 mentions) Tib 202, by American Type Culture Collection (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)

2 protocols using f8192 500ml

1

Colipterins Modulate IL-8 Secretion in THP-1 Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human THP-1 (ATCC TIB-202) cells were authenticated and purchased from ATCC, and no mycoplasma contamination was detected according to the manufacturer. The cells were cultured in RPMI medium (Gibco 11875093) with 10% FBS (Sigma F8192–500Ml) and 1% penicillin-streptomycin (Gibco 15070063) and were differentiated into macrophage-like cells by incubation in the presence of 50 nM PMA (Sigma P1585–1MG). Colipterins (17 μM, 1 μM, 0.1 μM, 0.01 μM in DMSO) or DMSO vehicle control (Santa Cruz 202581) were added 1 h prior to differentiation. 100 ng ml−1 LPS (Invivogen tlrl-peklps) were added after 16 h PMA incubation. After 24 h LPS treatment, culture supernatants were collected for IL-8 ELISA assays (R&D DY208–05) according to the manufacturer’s protocol. This assay was performed in triplicates.
Park H.B., Wei Z., Oh J., Xu H., Kim C.S., Wang R., Wyche T.P., Piizzi G., Flavell R.A, & Crawford J.M. (2020). Sulfamethoxazole drug stress upregulates antioxidant immunomodulatory metabolites in Escherichia coli. Nature microbiology, 5(11), 1319-1329.
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2

Colipterins Modulate IL-8 Secretion in THP-1 Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human THP-1 (ATCC TIB-202) cells were authenticated and purchased from ATCC, and no mycoplasma contamination was detected according to the manufacturer. The cells were cultured in RPMI medium (Gibco 11875093) with 10% FBS (Sigma F8192–500Ml) and 1% penicillin-streptomycin (Gibco 15070063) and were differentiated into macrophage-like cells by incubation in the presence of 50 nM PMA (Sigma P1585–1MG). Colipterins (17 μM, 1 μM, 0.1 μM, 0.01 μM in DMSO) or DMSO vehicle control (Santa Cruz 202581) were added 1 h prior to differentiation. 100 ng ml−1 LPS (Invivogen tlrl-peklps) were added after 16 h PMA incubation. After 24 h LPS treatment, culture supernatants were collected for IL-8 ELISA assays (R&D DY208–05) according to the manufacturer’s protocol. This assay was performed in triplicates.
Park H.B., Wei Z., Oh J., Xu H., Kim C.S., Wang R., Wyche T.P., Piizzi G., Flavell R.A, & Crawford J.M. (2020). Sulfamethoxazole drug stress upregulates antioxidant immunomodulatory metabolites in Escherichia coli. Nature microbiology, 5(11), 1319-1329.
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