Polyclonal anti laminin

The following antibodies were used: the anti-p-NFκB p65 (Ser536) (clone 9H31) and the anti-NFκB p65 (clone C22B4) from Cell Signaling Technology (Danvers, MA, USA); the anti-Gapdh (clone 6C5) from Santa Cruz Biotechnology (Dallas, Texas, USA); the anti-embryonic Myosin Heavy Chain, eMyHC (clone F1.652) from Developmental Studies Hybridoma Bank (Iowa City, IA, USA); polyclonal anti-Laminin from Sigma-Aldrich; the anti-CD45 PE/Cy5 conjugated (clone 30-F11) from BD Pharmigen™ (San Jose, CA, USA); the anti-CD31 Alexa Fluor 488 conjugated (clone MEC13.3) from Biolegend (San Diego, CA, USA); the anti-mouse IgG TRITC conjugated and the anti-rabbit IgG TRITC conjugated from Sigma Aldrich; the anti-mouse IgG Alexa Fluor 488 conjugated from Invitrogen; the anti-rabbit IgG Chromeo 488 conjugated from Abcam (Cambridge, MA, USA); the anti-mouse IgG and the anti-rabbit IgG HRP conjugated from Bethyl Laboratories (Montgomery, TX, USA).

Dewaxed sections were washed in phosphate buffered saline buffer, incubated in 1% BSA in TBS for 10 minutes before a further overnight incubation in primary antibody diluted in TBS. After washing, sections were incubated in biotin-conjugated or Alexa Fluor-conjugated secondary antibodies diluted in TBS and washed again in TBS. When biotinylated secondary antibodies were used, the sections were incubated with streptavidin peroxidase, and the staining reaction was performed using diaminobenzidine/H₂O₂. The following antibodies were used: polyclonal anti-phospho-histone H3 (1∶200; Millipore, catalogue number 06-570), polyclonal anti-Laminin (1∶200; Sigma, catalogue number L-9393), polyclonal anti-fibronectin (1∶200; Sigma, catalogue number F-3648) and polyclonal anti-salmon fish type I collagen (1∶1000; Novotec, catalogue number S20171). The secondary antibodies used were biotinylated goat anti-rabbit antibodies (Thermo scientific) and Alexa Fluor 488-conjugated goat anti-rabbit antibodies (1∶200; Invitrogen).