Ab66218

Manufactured by Abcam

Sourced in United States

Ab66218 is a mouse monoclonal antibody that targets the human KRT18 protein. It is suitable for use in applications such as Western blot, immunohistochemistry, and immunocytochemistry.

Automatically generated - may contain errors

Lab products found in correlation

Ab84296, by Abcam (1 mentions) Lab tek chambered coverglass, by Thermo Fisher Scientific (1 mentions) Dapi fluoromount g, by Southern Biotech (1 mentions) Egm 2 medium, by Lonza (1 mentions) Vimentin, by Zhongshan Golden Bridge Biotechnology (1 mentions) Ab113670, by Abcam (1 mentions) D9542, by Merck Group (1 mentions) Ds ril u2, by Nikon (1 mentions) Ab1316, by Abcam (1 mentions)

2 protocols using ab66218

Immunofluorescence Analysis of PDR Membranes

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IF staining was performed on 14 PDR membranes, which are seven samples from IVB treatment group, and seven samples from non-treatment group in 10 μm thick frozen sections. We used antibodies against the following antigens: TGFβ2 (1:200 dilution, ab113670, Abcam, Cambridge, Massachusetts, USA), CTGF (1:200 dilution, ab66218, Abcam), CNTF (1:200 dilution, ab66218, Abcam), VEGF (1:200 dilution, ab1316, Abcam) and vimentin (1:150 dilution, Zhongshan Golden Bridge Biotechnology, Beijing, China). Samples were counterstained with DAPI (1:1000 dilution, D9542, Sigma-Aldrich, USA). Sections were examined and photographed using a fluorescence microscope (DS-Ril-U2, Nikon, Tokyo, Japan).

Zhang Q., Qi Y., Chen L., Shi X., Bai Y., Huang L., Yu W., Jiang Y., Zhao M, & Li X. (2016). The relationship between anti-vascular endothelial growth factor and fibrosis in proliferative retinopathy: clinical and laboratory evidence. The British journal of ophthalmology, 100(10).

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Immunocytochemistry of APJ Receptor

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HREC cells were seeded on four chamber Lab-Tek Chambered Coverglass (Thermo Scientific; 1500 cells/chamber) for cell imaging in EGM-2 medium (Lonza) in a 5% CO₂ atmosphere at 37°C for 1 day. Chambers were washed with ice-cold PBS and fixed for 10 min with paraformaldehyde (4%) on ice then washed in PBS containing Triton X-100 (0.2% v/v) for 2 x 20 min to permeabilize the cells. To block nonspecific protein-protein interactions, cells were washed with Odyssey Blocking Buffer and PBS (1:1 v/v) containing 5% BSA for 1 h at room temperature then incubated with rabbit anti -APJ antibodies (Abcam; ab66218, ab84296, ab140508; 1:500 dilution, 2μg/ml) overnight at +4°C. Goat anti-rabbit Alexa Fluor 594 secondary IgG (H+L; Invitrogen, 1:500 dilution, 4μg/ml) and DAPI Fluoromount-G (SouthernBiotech) were used for anti-APJ receptor antibodies labeling and cell nuclei staining for confocal fluorescence microscopy detection.

McAnally D., Siddiquee K., Gomaa A., Szabo A., Vasile S., Maloney P.R., Divlianska D.B., Peddibhotla S., Morfa C.J., Hershberger P., Falter R., Williamson R., Terry D.B., Farjo R., Pinkerton A.B., Qi X., Quigley J., Boulton M.E., Grant M.B, & Smith L.H. (2018). Repurposing antimalarial aminoquinolines and related compounds for treatment of retinal neovascularization. PLoS ONE, 13(9), e0202436.

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