The largest database of trusted experimental protocols

Ap conjugated anti rabbit igg

Manufactured by Jackson ImmunoResearch
Sourced in United Kingdom, United States

AP-conjugated anti-rabbit IgG is a laboratory reagent that binds to rabbit immunoglobulin G (IgG) antibodies. The AP (Alkaline Phosphatase) conjugate allows for the detection and visualization of rabbit IgG in various immunoassay applications.

Automatically generated - may contain errors

3 protocols using ap conjugated anti rabbit igg

1

Western Blot Analysis of Embryonic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
For Western blot analysis, protein extracts prepared from the embryos were loaded in 12.5% or 15% sodium dodecyl sulfate polyacrylamide gel electrophoresis. The transferred membrane was incubated with anti-Rdd antibody or anti-HA antibody (Sigma, St. Louis, MO, USA) as a first antibody and was incubated with AP-conjugated anti-rabbit IgG (Jackson ImmunoResearch, Cambridge, UK) or PO-conjugated antimouse IgG (Santa Cruz Biotechnology, Santa Cruz, CA, USA) as a second antibody. Positive signals were visualized by NBT/BCIP solution or ECL Western Blotting Detection Reagent (GE Healthcare, Piscataway, NJ, USA) as a substrate.
+ Open protocol
+ Expand
2

Western Blot Analysis of SNAP-25

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chimeric MAb and rabbit sera were tested for specificity by Western blot. Lysates containing equal concentrations of total protein, as measured by the Bradford assay, were loaded to a NuPAGE 10% Bis-Tris Gel (Invitrogen, Carlsbad, CA, USA), and the electrophoresed proteins were transferred to an Amersham Protran Premium 0.45 µm nitrocellulose membrane (GE Healthcare 10600096). The membrane was then blocked (1% skim milk in PBS) and probed with chimeric MAb or immunized rabbit sera. Rabbit anti-SNAP-25 polyclonal antibody raised against a synthetic peptide corresponding to the N-terminus of human SNAP-25 (Sigma S9684), was used as a positive control to detect both the cleaved and the intact SNAP-25. AP-conjugated anti-human IgG (Sigma A3187) and AP-conjugated anti-rabbit IgG (Jackson 711-066-152) were used as secondary antibodies for chimeric MAb and rabbit sera, respectively. 5-Bromo-4-Chloro-3-Indolyl-phosphate (BCIP, Sigma B8503) was used as the chromogenic substrate. Densitometry analysis showed that the total SNAP-25 (cleaved and intact) in the BoNT/E treated samples produced similar signals, further confirming that equal amounts of the subject protein were loaded to the gels (Figure S1).
+ Open protocol
+ Expand
3

SARS-CoV-2 Spike Protein ELISA Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
A standard direct ELISA protocol was applied essentially as described [18 (link)]. Microtiter plates were coated using 0.1 μg/well of recombinant SARS-CoV-2 spike, S1 domain, RBD, NTD subunits or huACE2 (Sino Biological, Beijing, China). AP-conjugated anti-rabbit IgG or AP-conjugated anti-human IgG (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) were applied for rabbit sera or recombinant antibodies, respectively. Detection was performed using PNPP substrate (Sigma, Rehovot, Israel). Data points were fitted using non-linear regression (Prism 5, GraphPad, San Diego, CA, USA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!