LA/C-C epitope was mapped using custom-made PepSpot-membrane consisting of 61 peptides (residue 319 to 566, Lamin A/C, partially overlapping by 11 aa) (JPT Peptide Technologies GmbH, Berlin, Germany). In order to reduce any unspecific binding, the membrane was first incubated with the isotype control, mouse IgG1 kappa monoclonal (Abcam), 1:500 dilution, followed by HRP secondary (1:3000) incubation and ECL substrate developing. PepSpot-membrane washing and blocking steps followed the manufacture’s recommendations. In a second blotting step, the PepSpot membrane was incubated with the primary antibody of interest LA/C-C 1:1000, and further blotted using AP secondary antibody (1:3000) developed with CDPstar substrate.
Mouse igg1 kappa monoclonal
Manufactured by Abcam
Mouse IgG1 kappa monoclonal is a lab equipment product that functions as an antibody. It is a mouse-derived immunoglobulin G1 (IgG1) antibody with a kappa light chain.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mouse igg1 kappa monoclonal
1
Epitope Mapping of Lamin A/C Using PepSpot
2
Epitope Mapping of Lamin A/C Using PepSpot
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LA/C-C epitope was mapped using custom-made PepSpot-membrane consisting of 61 peptides (residue 319 to 566, Lamin A/C, partially overlapping by 11 aa) (JPT Peptide Technologies GmbH, Berlin, Germany). In order to reduce any unspecific binding, the membrane was first incubated with the isotype control, mouse IgG1 kappa monoclonal (Abcam), 1:500 dilution, followed by HRP secondary (1:3000) incubation and ECL substrate developing. PepSpot-membrane washing and blocking steps followed the manufacture’s recommendations. In a second blotting step, the PepSpot membrane was incubated with the primary antibody of interest LA/C-C 1:1000, and further blotted using AP secondary antibody (1:3000) developed with CDPstar substrate.
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