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Silica gel 60 high performance tlc plates

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Silica gel 60 high-performance TLC plates are a type of thin-layer chromatography (TLC) plates used for analytical separation and identification of compounds. They are composed of silica gel, a porous and inert material, which serves as the stationary phase for TLC. These plates provide high-performance separation capabilities due to the uniform and controlled pore size and particle distribution of the silica gel.

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Lab products found in correlation

γ 32p atp, by PerkinElmer (3 mentions) Everolimus rad001, by Selleck Chemicals (1 mentions)

Close spelling variants of this product

Silica gel 60 TLC plates Silica gel

3 protocols using silica gel 60 high performance tlc plates

1

Radiolabeled Kinase Assay Protocol

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Silica gel 60 high-performance TLC plates were from VWR (West Chester, PA, USA), and [γ-32P]-ATP was purchased from Perkin-Elmer (Waltham, MA, USA). Everolimus (RAD001) from Selleckchem (Newmarket, UK). All other chemicals were from Sigma Aldrich (Poole, UK).
Alshaker H., Wang Q., Kawano Y., Arafat T., Böhler T., Winkler M., Cooper C, & Pchejetski D. (2016). Everolimus (RAD001) sensitizes prostate cancer cells to docetaxel by down-regulation of HIF-1α and sphingosine kinase 1. Oncotarget, 7(49), 80943-80956.
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2

TLC Plate Preparation with Radiolabeled ATP

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Silica gel 60 high-performance TLC plates were purchased from VWR (West Chester, PA, USA), and [γ-32P]-ATP was purchased from Perkin-Elmer (Waltham, MA, USA). SK inhibitor SKI-II was purchased from Selleckchem (Newmarket, UK). All other chemicals were from Sigma Aldrich (Poole, UK).
Alshaker H., Srivats S., Monteil D., Wang Q., Low C.M, & Pchejetski D. (2018). Field template-based design and biological evaluation of new sphingosine kinase 1 inhibitors. Breast Cancer Research and Treatment, 172(1), 33-43.
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3

Radiolabeled Phosphorylation Assay

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Silica gel 60 high-performance TLC plates were from VWR (West Chester, PA, USA), and [γ-32P]-ATP was purchased from Perkin-Elmer (Waltham, MA, USA). RAD001 (everolimus) from Selleckchem (Newmarket, UK). Other reagents and chemicals used were purchased from Sigma-Aldrich (Dorset, UK) unless otherwise specified.
Alshaker H., Wang Q., Böhler T., Mills R., Winkler M., Arafat T., Kawano Y, & Pchejetski D. (2017). Combination of RAD001 (everolimus) and docetaxel reduces prostate and breast cancer cell VEGF production and tumour vascularisation independently of sphingosine-kinase-1. Scientific Reports, 7, 3493.
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