Waters xevo g2 xs q tof mass spectrometer

The HEK-293 cells transfected with FSHR and a coupled G protein were purchased from Merck Millipore (Milford, MA, USA). The GS HiRange kit was purchased from PerkinElmer Corp (Waltham, MA, USA). The Waters Xevo G2-XS Q-TOF mass spectrometer, equipped with an Acquity UPLC system, Acquity glycan BEH amide UPLC column and HPLC Alliance 2690 were purchased from Waters (Milford, MA, USA). The BIOSEP SEC-S2000 column was purchased from Tosoh Bioscience GmbH (Stuttgart, Germany). Amicon Ultra 3K centrifugal filters were purchased from Merck Millipore (Watford, UK). Water used in experiments was purified with a Milli-Q system from Merck Millipore (Milford, MA, USA). GlycoClean S cartridges were purchased from Prozyme Inc. (Hayward, CA, USA). The cell counter was purchased from Chemometec (Allerod, Denmark) and the homogeneous time-resolved fluorescence (HTRF) plate reader was purchased from Agilent (Santa Clara, CA, USA).

All the instrumentation used in this experiment included a double single-side purification workbench (Shanghai Shuo Guang Electronic Technology Corporation, Shanghai, China), Intelligent Biochemical Incubator (Shanghai Shuo Guang Electronic Technology Corporation, Shanghai, China), autoclave (Panasonic Healthcare Corporation, Tokyo, Japan), high-speed centrifuge (Eppendorf, Hamburg, Germany), Large-Capacity Combined Shock Incubator (Shanghai Minquan Instrument Corporation, Shanghai, China), FLEXA Purification System (Tianjin, China, Agela Technologies), Rotary Evaporator (Ningbo Hangjing Biotechnology Corporation, Ningbo, China), Waters Xevo G2-XS Q-Tof mass spectrometer (Waters Corporation, Milford, MA, USA), Agilent HPLC 1260 Infinity instrument (Agilent Technologies Inc., Santa Clara, CA, USA), and 600 MHz Bruker AVANCE NEO spectrometer (Bruker, Karlsruhe, Germany).