Manganese 2 chloride tetrahydrate mncl2

Anti-LAMP2 antibody was purchased from Santa Cruz Biotechnology (Dallas, USA). Polyclonal goat anti-mouse immunoglobulins HRP conjugated were purchased from Dako (Glostrup, Denmark). D-(+)-Galactose, zinc chloride (ZnCl₂) and nickel sulfate hexahydrate (NiSO₄) were purchased from Sigma (Saint Louis, USA), manganese (II) chloride tetrahydrate (MnCl₂) was from Riedel-de-Haën (Seelze, Germany), calcium chloride (CaCl₂) and iron (III) chloride (FeCl₃) were from ACROS Organics (New Jersey, USA), copper (II) sulphate (CuSO₄) was from Prolabo (France), lithium chloride (LiCl) was from Bio Basic Canada (Canada), magnesium chloride (MgCl₂) was from Euromedex (Souffelweyersheim, France) and iron (II) tetrahydrate (FeCl₂) was purchased from WVR Chemicals (Germany).

Anti-TMEM165 and anti-β-actin antibodies were purchased from Sigma-Aldrich (Burlington, MA, USA), anti-LAMP2 antibody from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-SPCA1 antibodies were purchased from Sigma-Aldrich to be used in immunofluorescence staining (IF) and from Biotechne (Minneapolis, MN, USA) to be used in western-blotting (WB), and anti-GM130 antibody was purchased from BD Biosciences (Franklin Lakes, NJ, USA). Polyclonal goat anti-rabbit or goat anti-mouse horseradish peroxydase-conjugated Igs were from Agilent Technologies (Santa Clara, CA, USA). Polyclonal goat anti-mouse or goat anti-rabbit conjugated with Alexa Fluor were purchased from Fisher Scientific (Waltham, MA, USA). Manganese (II) chloride tetrahydrate (MnCl₂) was from Riedel-de-Haën (Seelze, Germany). Digitonin was purchased from Fisher Scientific (Waltham, USA).