Lyve 1 egfp cre

Homozygous Kimba (vegfa^+/+) mice were mated with Lyve1-positive mice (Lyve1^EGFP/Cre) (012601; Jackson Laboratory, Bar Harbor, ME, USA), to generate Lyve1-positive Kimba (Lyve1^EGFP/Cre vegfa^+/−) mice.

CX3CR1^GFP/GFP, CCR2^GFP/GFP, Lyve1^EGFP-Cre, Cx3cr1^CreER, and R26^Td/DTR mice were purchased from the Jackson Laboratory. Male wild-type (WT) mice (C57BL/6, 3 to 4 weeks old) from Model Organisms Center Inc. (Shanghai, China) were used in this study. CX3CR1^GFP/+ mice were generated by crossing CX3CR1^GFP/GFP mice with WT mice. Similarly, CCR2^GFP/GFP mice were crossed with WT mice to generate CCR2^GFP/+ mice. To obtain Cx3cr1^CreER×R26^Td/DTR mice, Cx3cr1^CreER mice were crossed with R26^Td/DTR mice. The Cx3cr1^CreER×R26^Td/DTR mice exhibited selective depletion of Td⁺ cells following administration of DT. Because previous studies demonstrated a protective effect of estrogen against ischemic cardiac injury and therefore suggested the male sex as an important risk factor for cardiovascular disease (46 (link)), only male mice were used in this study. This study and all the animal experimental procedures followed the Institutional Animal Care and Use Committee guidelines of Fudan University.