Phospho iκbα ser32 14d4 rabbit mab

Lipopolysaccharides (LPSs, from Escherichia coli serotype 026: B6) were from Sigma Chemical Co. (St. Louis, MO, USA). The CCK-8 Kit, ROS Assay Kit, T-AOC Assay Kit, Lipid Peroxidation MDA Assay Kit, Griess Reagent, ELISA kits for TNF-α, IL-1β, IL-6, and IL-10 were from Beyotime Biotechnology Co. (Shanghai, China). Anti-TLR4 antibody (ab13556), anti-NF-κB p65 antibody (ab32536), anti-NF-κB p65 (phospho S536) antibody (ab76302), anti-beta actin antibody (ab8227), and secondary antibody (ab97051) were from Abcam (Cambridge, MA, USA). Human/Mouse/Rat Keap1 antibody (MAB3024) and Human/Mouse Nrf2 antibody (AF3925) were from R&D Systems (Minneapolis, MI, USA). IκBα (44D4) Rabbit mAb and Phospho-IκBα (Ser32) (14D4) Rabbit mAb were from Cell Signaling Technology (Beverly, MA, USA).

Media and serum were purchased from Invitrogen (Paisley, UK). Antibodies were sourced as follows: FADD mouse mAb (610400) was from BD Transduction Laboratories (San Jose, CA, USA); GST tag mouse mAb (71097) was from Novagen (Watford, UK); cleaved caspase-3 (Asp175) (9661) rabbit polyclonal antibody, cleaved caspase-8 (Asp384) (11G10) mouse mAb, Bid (2002) rabbit polyclonal antibody, Phospho-IκBα (Ser32) (14D4) rabbit mAb and total IκBα (44D4) rabbit mAb were sourced from Cell Signaling Technology Inc. (Hitchin, UK); caspase-8 rabbit polyclonal antibody was generated in our laboratory; caspase-8 mouse mAbs (C15 and N2) were kind gifts from Prof. PH Krammer (German Cancer Research Center, Heidelberg, Germany); the p85α and pan-Phospho-tyrosine (2C8) mouse mAbs were from Santa Cruz Biotechnology, Inc. (Middlesex, UK); Phospho (Tyr380) caspase-8 was a kind gift from Prof. D Barila (University of Rome Tor Vergata, Rome, Italy). Horseradish peroxidase-conjugated secondary antibodies were obtained from Sigma-Aldrich (goat anti-mouse; Dorset, UK) and DAKO (goat anti-rabbit; Cambridge, UK). The caspase-8 substrate, Ac-Ile-Glu-Thr-Asp-amino-4-trifluoromethyl coumarin (Ac-IETD.AFC) was purchased from MP Biomedicals (Cambridge, UK). All other chemicals were of analytical grade and obtained from Sigma-Aldrich or Fisher (Loughborough, UK).