To assess correlations among mRNA levels, we collected pathologically confirmed LUAD samples from 59 patients who underwent resections at the Johns Hopkins Hospital (Baltimore, MD, USA), the Johns Hopkins Bayview Medical Center (Baltimore, MD, USA), and the Medical College of Wisconsin Froedtert Memorial Hospital (Milwaukee, WI, USA) (20 (link)). Formalin-fixed paraffin-embedded TMA sections were constructed from blocks of resected specimens from 165 LUAD patients who were treated at the Sacro Cuore Don Calabria Hospital (Negrar, Verona, Italy) (21 ). None of the patients received any therapies before their resections. Antibodies for YAP1 (EP1674Y; Abcam, Cambridge, UK; #ab52771; 1:300) and pSTAT3 (D3A7; Cell Signaling Technology; #9145; 1:25) were used for IHC, which were scored blindly by one pathologist (Dr Gabrielson). Intensity scores (0; none, 1+; weak, 2+; moderate, 3+; strong) of nuclei staining were evaluated by light microscopy; a score of 0 was considered negative, and scores of 1+, 2+ or 3+ were considered positive.
Ep1674y
Manufactured by Abcam
Sourced in United Kingdom
EP1674Y is an anti-PARP1 antibody produced in rabbit. It is intended for use in Western blot and immunohistochemistry applications.
Automatically generated - may contain errors
Lab products found in correlation
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Ab11805, by Abcam (1 mentions)
Ab52771, by Abcam (1 mentions)
Ecl reagent, by GE Healthcare (1 mentions)
Sc 32233, by Santa Cruz Biotechnology (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
Vimentin, by BD (1 mentions)
Hsp90, by BD (1 mentions)
2 protocols using ep1674y
1
LUAD Pathology: Correlating mRNA Levels
2
Western Blot Analysis of Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were harvested by scraper and lysed with RIPA buffer supplemented with 1× complete protease inhibitor cocktail (Roche) following the manufacturer’s protocol. Protein concentrations were determined using a Pierce BCA protein assay kit (Thermo Scientific). Lysates were separated on SDS-PAGE gels and transferred. Membranes were immunoblotted with the following antibodies: YAP (ab52771, EP1674Y, abcam; 1:1000), TRAM2 (ab109176, EPR2658, abcam; 1:5000), FSTL-1 (ab11805, abcam, 1:1000), Vimentin (550,513, BD Biosciences, 1:2000), HSP90 (610,418, BD Biosciences, 1:5000), and GAPDH (sc-32,233, Santa Cruz, 1:2000). Protein bands were visualized using corresponding secondary antibodies (Dako) and ECL reagent (GE Healthcare).
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