Quantification of SL was performed using an Agilent Infinity 1290 UHPLC system QQQ (Agilent Technologies, Santa Clara, CA, USA) with a diode array detector. In a Phenomenex Kinetex C18 (150*2.1 mm, 2.6 μm) column. The gradient was linear with water and acetonitrile (both buffered with 20 mM formic acid) starting at 10% at a flow rate of 0.59 mL/min and ending at 10 min (100%). The ion source was Agilent Jet Stream Electrospray Ionization with Dynamic MRM as scan type and an injection volume of 1 μL was used. MS detection was performed on an Agilent 6490 Triple Quad MS equipped with Agilent Dual Jet Stream electrospray ion source with Dynamic MRM. Identification of individual compounds was based on findings in Graziani et al.20 (link). Standards used for quantification was lactucin (3809, Extrasynthese SAS, 69727 GENAY Cedex) and 11beta,13-dihydrolactucopicrin (3811, Extrasynthese SAS, 69727 GENAY Cedex). A dilution curve of 0.1, 1, 10 and 100 ug/mL was used to calculate the equation.
6490 triple quad ms
Manufactured by Agilent Technologies
The 6490 Triple Quad MS is a high-performance mass spectrometer designed for quantitative and qualitative analysis. It features a triple quadrupole configuration for enhanced sensitivity and selectivity. The core function of this instrument is to provide accurate and reliable mass spectral data for a wide range of analytical applications.
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3 protocols using 6490 triple quad ms
1
Quantification of Sesquiterpene Lactones via UHPLC-QQQ
2
Quantification of Sesquiterpene Lactones in UHPLC-MS
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Quantification of SL was performed using an Agilent Infinity 1290 UHPLC system QQQ (Agilent Technologies, Santa Clara, CA, USA) with a diode array detector. The column used was a Phenomenex Kinetex C18 (150*2.1 mm, 2.6 μm). The gradient was linear with water (A) and acetonitrile (B) (both buffered with 20 mM formic acid) starting at 10% B at a flow rate of 0.59 mL/min and ending at 10 min (100% B). The ion source was Agilent Jet Stream Electrospray Ionization with Dynamic MRM as scan type. An injection volume of 1 μL was used. MS detection was performed in positive ion mode on an Agilent 6490 Triple Quad MS equipped with Agilent Dual Jet Stream electrospray ion source with Dynamic MRM as scan type. Identification of individual compounds was based on previous findings (Graziani et al., 2015 ). Standards used for quantification of SL were lactucin (3809, Extrasynthese SAS, 69727 GENAY Cedex) and 11beta,13-dihydrolactucopicrin (3811, Extrasynthese SAS, 69727 GENAY Cedex). A dilution curve of 0.1, 1, 10 and 100 parts per million (PPM) was used to calculate the equation.
3
Nucleotide Sugar Analysis by LC-MS
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Nucleotide sugars were analyzed by reversed-phase ionpairing chromatography (Agilent Technologies 1290 Infinity LC) with a HSS T 3 column (Waters, 2.1 ϫ 150 mm i.d., 1.8 m particle size) coupled to a triple quadrupole mass spectrometer operating in negative ion mode (Agilent Technologies 6490 Triple Quad MS) (see Materials file in the online Data Supplement).
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