Immunofluorescence of cryosections and EREC were analyzed by confocal microscopy (Leica TCS SP2 Laser Scanning Spectral Confocal System; Leica Microsystems). A Gre-Ne 543 nm laser was used to excite Texas Red-fluorochromes. An Argon 488 nm laser excited FITC-fluorochromes.
Tcs sp2 laser scanning spectral confocal system
Manufactured by Leica
The TCS SP2 Laser Scanning Spectral Confocal System is a high-performance microscopy tool designed for advanced imaging and analysis. It features a multi-wavelength laser system, a spectral detector, and a confocal scanning unit to provide high-resolution, multi-dimensional imaging capabilities.
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2 protocols using tcs sp2 laser scanning spectral confocal system
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Confocal Microscopy of Immunofluorescence
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Confocal Imaging of Immunofluorescence
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Immunofluorescence staining of all cells was analyzed by confocal microscopy (Leica TCS SP2 Laser Scanning Spectral Confocal System; Leica Microsystems). A Gre-Ne 543 nm laser was used to excite Texas Red-fluorochromes. An Argon 488 nm laser excited FITC-fluorochromes.
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