Anti rraga

Manufactured by Cell Signaling Technology

Anti-RRAGA is a primary antibody that recognizes the RRAGA protein. RRAGA is a member of the Ras-related GTP-binding protein family and plays a role in regulating the activity of the mTOR signaling pathway.

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2 protocols using anti rraga

Generation and Characterization of RRAGC Mutants

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A pCMV-SPORT plasmid containing the RRAGC cDNA (cat#: MHS6278-202757712; accession: BC016668) was purchased from ThermoScientific, and used as a template to generate mutant RRAGC cDNAs using the QuikChange® Lightning Site-Directed Mutagenesis Kit (Stratagene/Agilent, La Jolla, CA). Full-length wild type and mutant HA-tagged RRAGC were constructed using PCR and cloned into the PacI/NotI sites of the lentiviral vector FG9 (a gift from Dr. Colin Duckett, University of Michigan(32 (link))). HA-tagged raptor (#8513) and RRAGB WT (#19301) was purchased from Addgene (Cambridge, MA)(27 (link)). Anti-HA antibody was purchased from Roche, Basel Switzerland (clone 3F10, #11867423001). Anti-raptor (05–1470) was from Millipore. Anti-beta-Actin, (#A544) was from Sigma Aldrich. Antibodies against RRAGC (#3360), anti-folliculin (#3697), anti-RRAGB (#8150), anti-RRAGA (#4357), anti-S6Kinase (#9202) and anti-pS6-Kinase-Thr 389 (#9205) were purchased from Cell Signaling Technology (Danvers, MA).

Ying Z.X., Jin M., Peterson L.F., Bernard D., Saiya-Cork K., Yildiz M., Wang S., Kaminski M.S., Chang A.E., Klionsky D.J, & Malek S.N. (2016). Recurrent mutations in the MTOR regulator RRAGC in follicular lymphoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(21), 5383-5393.

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Immune Receptor Signaling Pathway Analysis

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ssRNA40, R848, and other PRR ligands were from InvivoGen, phytohemagglutinin from Rembrel. Anti‐FLAG, anti‐EEA1, anti‐Rab7, anti‐SHP1, anti‐RRAGA, anti‐STAT1, anti‐Phospho‐STAT1, anti‐IkBα, anti‐pp38, anti‐phospho‐Erk were from Cell Signaling; anti‐LAMP1, anti‐beta‐tubulin, anti‐beta‐actin were from Abcam; anti‐phosphotyrosine (PY20) was from Zymed, anti‐HIV‐1 p18 (4C9) was from NIBSC. Anti‐CD4 and anti‐CD3 were from BD biosciences, anti‐HIV‐1 core antigen (KC57) was from Beckman Coulter, Violet LIVE/DEAD Cell Stain was from Invitrogen. GABARAP was from Abnova, PSMB6 and SNX5 were from Santa Cruz, BLOC1S1 was from Proteintech, Snapin was from Synaptic Systems. Anti‐rabbit Alexa Fluor 555, anti‐mouse Alexa Fluor 647, and streptavidin‐coated Dynabeads were from Invitrogen, pHrodo from Thermo Fisher, anti‐mouse HRP and anti‐goat HRP were from Sigma.

Khatamzas E., Hipp M.M., Gaughan D., Pichulik T., Leslie A., Fernandes R.A., Muraro D., Booth S., Zausmer K., Sun M., Kessler B., Rowland‐Jones S., Cerundolo V, & Simmons A. (2017). Snapin promotes HIV‐1 transmission from dendritic cells by dampening TLR8 signaling. The EMBO Journal, 36(20), 2998-3011.

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