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Non glucosylated rac1 clone 102

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Non-glucosylated RAC1 (Clone 102) is a laboratory reagent that provides the core function of the Ras-related C3 botulinum toxin substrate 1 (RAC1) protein in its non-glucosylated state. This recombinant protein is expressed and purified for use in research applications.

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Lab products found in correlation

β actin ac 15, by Merck Group (2 mentions) Hela h1, by American Type Culture Collection (2 mentions) Cho c6, by American Type Culture Collection (2 mentions) Rac1 23a8, by Abcam (2 mentions) Rabbit monoclonal igg against ldlr ep1553y, by Abcam (2 mentions) Ht 29, by American Type Culture Collection (2 mentions) Originpro 8, by OriginLab (2 mentions)

2 protocols using non glucosylated rac1 clone 102

1

Cell Lines and Antibody Sources

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa (H1, #CRL-1958), HT-29 (#HTB-38), CHO-C6, and 293T (#CRL-3216) cells were originally obtained from ATCC. They tested negative for mycoplasma contamination, but have not been authenticated. Huh7 and Huh7 LDLR−/− cells were kindly provided by Y. Matsuura36 (link). The following mouse monoclonal antibodies were purchased from the indicated vendors: RAC1 (23A8, Abcam), non-glucosylated RAC1 (Clone 102, BD Biosciences), β-actin (AC-15, Sigma), and HS (F58–10E4, mouse IgM, Amsbio). Rabbit monoclonal IgG against LDLR (EP1553Y) was purchased from Abcam. Chicken polyclonal IgY (#753A) against TcdA was purchased from List Biological Labs. Statistical analysis was performed using OriginPro 8 (V8.0724, OriginLab Corp.) software.
Tao L., Tian S., Zhang J., Liu Z., Robinson-McCarthy L., Miyashita S.I., Breault D.T., Gerhard R., Oottamasathien S., Whelan S, & Dong M. (2019). Sulfated glycosaminoglycans and low-density lipoprotein receptor contribute to Clostridium difficile toxin A entry into cells. Nature microbiology, 4(10), 1760-1769.
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2

Cell Lines and Antibody Sources

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa (H1, #CRL-1958), HT-29 (#HTB-38), CHO-C6, and 293T (#CRL-3216) cells were originally obtained from ATCC. They tested negative for mycoplasma contamination, but have not been authenticated. Huh7 and Huh7 LDLR−/− cells were kindly provided by Y. Matsuura36 (link). The following mouse monoclonal antibodies were purchased from the indicated vendors: RAC1 (23A8, Abcam), non-glucosylated RAC1 (Clone 102, BD Biosciences), β-actin (AC-15, Sigma), and HS (F58–10E4, mouse IgM, Amsbio). Rabbit monoclonal IgG against LDLR (EP1553Y) was purchased from Abcam. Chicken polyclonal IgY (#753A) against TcdA was purchased from List Biological Labs. Statistical analysis was performed using OriginPro 8 (V8.0724, OriginLab Corp.) software.
Tao L., Tian S., Zhang J., Liu Z., Robinson-McCarthy L., Miyashita S.I., Breault D.T., Gerhard R., Oottamasathien S., Whelan S, & Dong M. (2019). Sulfated glycosaminoglycans and low-density lipoprotein receptor contribute to Clostridium difficile toxin A entry into cells. Nature microbiology, 4(10), 1760-1769.
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