Daughterless gal4

Manufactured by BD

The Daughterless-GAL4 is a genetic tool used in Drosophila (fruit fly) research. It functions as a transcriptional activator, driving the expression of target genes in a specific manner.

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Lab products found in correlation

Gmr gal4, by BD (1 mentions) Actin gal4, by BD (1 mentions) Elav gal4, by BD (1 mentions) Tubulin gal4, by BD (1 mentions) Uas dmyc, by BD (1 mentions)

2 protocols using daughterless gal4

Drosophila Cytochrome P450 RNAi Screening

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Flies were raised and maintained on standard cornmeal-yeast medium under a 12/12 h light–dark cycle at 25 °C. The fly stocks used were obtained either from the Vienna Drosophila Resource Center (VDRC, www.vdrc.at), or the Bloomington Drosophila Stock Center (BDSC, www.bdsc.indiana.edu). Strains with GAL4-dependent expression of short hairpins (sh) to induce gene silencing via RNA interference (RNAi) of the following genes were used: sh-Cyp4ac1 (BDSC #67005), sh-Cyp307a2 (VDRC #330647), sh-Cyp18a1 (BDSC #64923), sh-Cyp9f2 (BDSC #67806), sh-Cyp4d2 (BDSC #42600), sh-Cyp4d1 (BDSC #52979), sh-Cyp311a1 (BDSC #67792), sh-Cyp312a1 (VDRC #101231), sh-Cyp28d2 (BDSC #61282), sh-Cyp28d1 (BDSC #53892), sh-Cyp4d21 (BDSC # 29238), sh-Cyp28a5 (BDSC #77405), sh-4d20 (BDSC #77341), sh-Cyp4s3 (BDSC #66978), sh-Cyp4c3 (BDSC #64213), h-Cyp304-a1 (BDSC #67805). Canton-S (BDSC #64349) served as wild type control. GAL4 drivers used to activate UAS-controlled expression of above mentioned sh-lines were actin-GAL4 (BDSC #4414) and daughterless-GAL4 (BDSC #55850) for ubiquitous expression. The driver elav-GAL4 (BDSC #458) was used to induce pan neural expression and/or GMR-GAL4 (BDSC #1104) to induce retina specific expression of transgenes under UAS-control. UAS-Apoliner (BDSC #32122) was used to detect caspase activation in flies after PCB 28 treatment.

Idda T., Bonas C., Hoffmann J., Bertram J., Quinete N., Schettgen T., Fietkau K., Esser A., Stope M.B., Leijs M.M., Baron J.M., Kraus T., Voigt A, & Ziegler P. (2020). Metabolic activation and toxicological evaluation of polychlorinated biphenyls in Drosophila melanogaster. Scientific Reports, 10, 21587.

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Drosophila Sirt6 Transgenic Lines

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dSirt6 lines were made by cloning the coding DNA sequence of Drosophila Sirt6 (CG6284) into the following constructs: a pUASt-based pTW vector (Drosophila Genomics Resource Center) for “UAS-Sirt6,” pTGW for “UAS-Sirt6-GFP,” and pUASg.attB for “UAS-Sirt6-3R.” UAS-Sirt6 and UAS-Sirt6-GFP were injected (Best Gene) into w¹¹¹⁸ flies to create transgenic lines, with insertions on the second chromosome, while UAS-Sirt6-3R was injected into M{3xP3-RFP.attP}ZH-86Fb flies (Bloomington Drosophila Stock Center [BDSC] #24749) to generate a site-specific insertion on the third chromosome. The following additional stocks were obtained from the BDSC: UAS-dMyc (BDSC #9674), dMyc⁴ (BDSC #64769), tubulin-GAL4 (BDSC #5138), EP-Sirt6 (BDSC #30115), UAS-Sirt6-RNAi (BDSC #34530), s106 (BDSC #8151), and daughterless-GAL4 (BDSC #55850). The tubulinGeneSwitch (“tubGS”) was a kind gift from S. Pletcher, University of Michigan, Ann Arbor, MI.
For all experiments, flies were aged and maintained on 15% dextrose/15% yeast/2% agar food at 25 °C on a 12-h light/dark cycle at 60% relative humidity. For experiments using GeneSwitch drivers, either RU486 dissolved in EtOH at the indicated concentration (Dataset S1) or EtOH alone (for controls) was added to the food at 20 mL/L.

Taylor J.R., Wood J.G., Mizerak E., Hinthorn S., Liu J., Finn M., Gordon S., Zingas L., Chang C., Klein M.A., Denu J.M., Gorbunova V., Seluanov A., Boeke J.D., Sedivy J.M, & Helfand S.L. (2022). Sirt6 regulates lifespan in Drosophila melanogaster. Proceedings of the National Academy of Sciences of the United States of America, 119(5), e2111176119.

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