Log In Sign up
The largest database of trusted experimental protocols

Chip grade tead4 antibody

Manufactured by Abcam
Visit Supplier
Sourced in United States

ChIP grade TEAD4 antibody is a laboratory reagent designed for use in chromatin immunoprecipitation (ChIP) experiments. It is specific to the TEAD4 protein, a transcription factor involved in various cellular processes.

Automatically generated - may contain errors

Lab products found in correlation

Magna chip protein a g magnetic beads, by Merck Group (2 mentions) Pcr purification kit, by Qiagen (2 mentions)

Close spelling variants of this product

ChIP Grade TEAD4

2 protocols using chip grade tead4 antibody

1

TEAD4 Chromatin Immunoprecipitation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were crosslinked in 0.75% formaldehyde for 15 min, then glycine was added to a final concentration of 125 mM for 5 min. After wash with cold PBS, cells were collected in PBS and sonicated on an ultrasonic homogenizer for 10 min at 20% power on ice to shear DNA to an average fragment size of 200–1000 bp. Fifty μL of each sonicated sample was removed to determine DNA concentration and fragment size. Cell lysates were incubated overnight with 20 μL Magna ChIP™ Protein A+G Magnetic Beads (EMD Millipore, Burlington, MA, USA) and 10 µg ChIP grade TEAD4 antibody (Abcam) at 4°C. Beads were collected, washed and treated with Proteinase K for 2 h at 60°C and RNase for 1 h at 37°C. DNA was purified with a PCR purification kit (Qiagen, Germantown, MD, USA). DNA fragments were assessed by qRT–PCR using the primer sequences listed in Supplementary Table 2. Samples were normalized to input DNA.
Wu J., Minikes A.M., Gao M., Bian H., Li Y., Stockwell B.R., Chen Z.N, & Jiang X. (2019). Intercellular interaction dictates cancer cell ferroptosis via Merlin-YAP signalling. Nature, 572(7769), 402-406.
+ Open protocol
+ Expand
2

TEAD4 Chromatin Immunoprecipitation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were crosslinked in 0.75% formaldehyde for 15 min, then glycine was added to a final concentration of 125 mM for 5 min. After wash with cold PBS, cells were collected in PBS and sonicated on an ultrasonic homogenizer for 10 min at 20% power on ice to shear DNA to an average fragment size of 200–1000 bp. Fifty μL of each sonicated sample was removed to determine DNA concentration and fragment size. Cell lysates were incubated overnight with 20 μL Magna ChIP™ Protein A+G Magnetic Beads (EMD Millipore, Burlington, MA, USA) and 10 µg ChIP grade TEAD4 antibody (Abcam) at 4°C. Beads were collected, washed and treated with Proteinase K for 2 h at 60°C and RNase for 1 h at 37°C. DNA was purified with a PCR purification kit (Qiagen, Germantown, MD, USA). DNA fragments were assessed by qRT–PCR using the primer sequences listed in Supplementary Table 2. Samples were normalized to input DNA.
Wu J., Minikes A.M., Gao M., Bian H., Li Y., Stockwell B.R., Chen Z.N, & Jiang X. (2019). Intercellular interaction dictates cancer cell ferroptosis via Merlin-YAP signalling. Nature, 572(7769), 402-406.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!