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Anti flag or myc antibody beads

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Anti-Flag or myc antibody beads are lab equipment used for protein purification. They consist of agarose beads coated with antibodies specific to the Flag or myc epitope tags commonly used in recombinant protein expression systems. These beads can be used to capture and purify proteins of interest that have been engineered to contain the corresponding epitope tag.

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Lab products found in correlation

Cellfectin 2, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Anti-FLAG (2121 citations) Anti-FLAG antibody (1016 citations) Anti-Myc (274 citations) Flag antibody (199 citations) Anti-FLAG beads (153 citations) Flag beads (126 citations) Anti-Myc antibody (109 citations) Anti-Myc beads (15 citations) Anti-Myc or anti-Flag antibody (2 citations)

2 protocols using anti flag or myc antibody beads

1

Affinity Purification of Protein Complexes

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293 cells were transfected with DNA constructs (made in pcDNA 3.1(+); primers listed in Supplementary Table 4) using Lipofectamine2000 (Life Technologies). The Drosophila S2 cells were transfected with DNA constructs (made in pAMW or pAFW from Drosophila Genome Resources Center; primers listed in Supplementary Table 4) using Cellfectin II (Life Technologies). Twenty-four to forty-eight hours after transient transfection, the cells were washed and extracted with 300 mM KCl buffer D. Extract (diluted to 150 mM KCl) were incubated with anti-flag or myc antibody beads (Sigma-Aldrich) for 2 hours, washed with 0.05% T/PBS, and eluates collected for analysis.
Peng J.C., Valouev A., Liu N, & Lin H. (2016). Piwi maintains germline stem cells and oogenesis in Drosophila through negative regulation of Polycomb Group proteins. Nature genetics, 48(3), 283-291.
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2

Affinity Purification of Protein Complexes

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293 cells were transfected with DNA constructs (made in pcDNA 3.1(+); primers listed in Supplementary Table 4) using Lipofectamine2000 (Life Technologies). The Drosophila S2 cells were transfected with DNA constructs (made in pAMW or pAFW from Drosophila Genome Resources Center; primers listed in Supplementary Table 4) using Cellfectin II (Life Technologies). Twenty-four to forty-eight hours after transient transfection, the cells were washed and extracted with 300 mM KCl buffer D. Extract (diluted to 150 mM KCl) were incubated with anti-flag or myc antibody beads (Sigma-Aldrich) for 2 hours, washed with 0.05% T/PBS, and eluates collected for analysis.
Peng J.C., Valouev A., Liu N, & Lin H. (2016). Piwi maintains germline stem cells and oogenesis in Drosophila through negative regulation of Polycomb Group proteins. Nature genetics, 48(3), 283-291.
+ Open protocol
+ Expand

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