Poly dimethyl siloxane pdms kit sylgard 184

Brij® S 100 polyoxyethylene (100) stearyl ether [PEG octadecyl ether, C₁₈H₃₇(OCH₂CH₂)_nOH, n ≈ 100, average Mn ≈ 4,670 Da], pyridine, gelatin from porcine skin, poly(D,L-lactide-co-glycolide) acid terminated (PLGA, lactide : glycolide = 75 : 25, Mw ≈ 4 – 15 kDa), thiazolyl blue tetrazolium bromide (MTT), bovine serum albumin (BSA), (3-aminopropyl)trimethoxysilane (APTES), succinic anhydride, N-hydroxysuccinimide (NHS), N,N’-dicyclohexylcarbodiimide (DCC), and CDCl₃ were purchased from Sigma-Aldrich. Poly(allylamine hydrochloride) (PAH) with Mw of 120 – 200 kDa, poly(styrene sulfonic acid) sodium salt (PSS) with Mw of 70 kDa, retinoic acid, trypsin (0.25%) EDTA, fetal bovine serum (FBS), and minimum essential medium eagle-alpha modification (α-MEM) were purchased from VWR. Poly(dimethyl siloxane) (PDMS) kit (Sylgard 184) was purchased from Dow Corning. Poly(n-propyl methacrylate) (PPMA) with Mw of 150 kDa was purchased from Scientific Polymer. Di-sodium tetraborate/sodium hydroxide buffer solution (pH 10) was purchased from Honeywell. Dulbecco’s modified eagle’s medium (DMEM, with 4.5 g/L glucose and 4 mM L-glutamine) was purchased from Lonza Bioscience. Octadecyl rhodamine B chloride (R18) and fluorescein isothiocyanate (FITC) were purchased from Biotium. Poloxamer 407 was purchased from Spectrum. NHS-functionalized PEG (PEG-NHS, Mw = 5 kDa) was purchased from Nanocs Inc.

Poly(n-isopropylacrylamide) (PNIPAM, M_n 40 kDa), poly(vinyl alcohol) (PVA, 88% hydrolyzed, molecular weight (MW) =3 kDa), fluorescein O-acrylate, 2,2’-azobis(isobutyronitrile) (AIBN), dextran-fluorescein isothiocyanate (dextran-FITC, MW = 3-5 kDa), lipopolysaccharides (LPS) from Escherichia coli O111:B4, and zymosan (zymosan A from saccharomyces cerevisiae) were purchased from MilliporeSigma. n-isopropylacrylamide (NIPAM) was purchased from TCI America. Fetal bovine serum (FBS) was purchased from VWR. 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine (DiI), 3,3'-dioctadecyloxacarbocyanine (DiO) and LysoView™ 488 (Lysoview) were purchased from Biotium. Carboxyfluorescein succinimidyl ester (CFSE) was purchased from ThermoFisher. Hoechst 33342 was purchased from Life Technologies. Poly(dimethyl siloxane) (PDMS) kit (Sylgard 184) was purchased from Dow Corning. Poly(n-propyl methacrylate) (PPMA) with a Mw of 150 kDa was purchased from Scientific Polymer Products. RAW264.7 macrophages were purchased from the American Type Culture Collection. Dulbecco’s modified eagle’s medium (DMEM, with 4.5 g/L glucose and 4 mM L-glutamine) was purchased from Lonza Bioscience. Poly(3-hexylthiophene-2,5-diyl) (P3HT, MW = 58 kDa) was purchased from Rieke Metals. Tetrahydrofuran (THF) was purchased from Merk.