Factor xa cleavage kit

Yeast extract and curcumin were obtained from Acros Organics (Geel, Belgium). Tryptic soy agar and gold(III) chloride trihydrate were acquired from MP Biomedicals (Santa Ana, CA). Ampicillin, isopropyl β-D-1-thiogalactopyranoside (IPTG), imidazole, sodium monobasic phosphate, sodium dibasic phosphate, sodium dodecyl sulfate, sodium hydroxide, sodium chloride, sucrose, tris-hydrochloride, tryptone, PFU high fidelity, DpnI, ACS grade methanol and urea were obtained from Fisher Scientific (Pittsburgh, PA). 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), magnesium sulfate, nickel chloride, sodium borohydride were purchased from Sigma Aldrich (St. Louis, MO). Tricine was purchased from Alfa Aesar (Ward Hill, MA). Glacial acetic acid and Factor Xa cleavage kit were purchased from EMD Millipore (Rockland, MA). Ethyl acetate was purchased from Pharmco-AAPER (Brookfield, CT). Ethylenediaminetetraacetic acid (EDTA) and hydrochloric acid were acquired from VWR (Radnor, PA). HPLC grade methanol was obtained from Ricca Chemical Company (Arlington, TX). Sephadex™ G-25 medium beads were purchased from Amersham Pharmacia Biotech AB (Piscataway, NJ). Columns were purchased from Bio-Rad (Hercules, CA).