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2 protocols using iron 2 chloride anhydrous

1

Cytotoxicity Assay of Metal-based Compounds

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Copper(II) chloride dehydrate (CuCl2·2H2O), hydrochloric acid (HCl, 37%), nitric acid (HNO3, 65%), and Dulbecco’s modified Eagle’s medium-high glucose (DMEM/HG) were purchased from Thermo Fisher Scientific (Massachusetts, USA). Iron(II) chloride anhydrous (FeCl2, 99.5%), methylene blue (C16H18ClN3) and thiazolyl blue tetrazolium bromide (MTT) were from Alfa Aesar (Massachusetts, USA). Hydrazine hydrate (N2H4·H2O) and sodium pyruvate (C3H3NaO3S·xH2O) were from Acros Organics (Morris Plains, NJ, USA). poly(styrene-alt-maleic acid) sodium salt solution (PSMA), hydrogen peroxide (H2O2), 2′,7′-dichlorofluorescein diacetate (DCFH-DA), sodium bicarbonate (NaHCO3), trypan blue (0.4%), imidazole, N,N-dimethyl-4-nitrosoaniline (RNO), N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), and folic acid (C19H19N7O6) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS), trypsin-ethylenediaminetetraacetic acid(trypsin-EDTA, 0.25%) and antibiotic-antimycotic (penicillin/streptomycin/amphotericin B) were from Biological Industries (Cromwell, CT, USA). Dimethyl sulfoxide was from Scharlau (Barcelona, Spain).
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2

Colorimetric Redox Reaction Assay

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Copper (II) chloride dehydrate (Riedel-de Haën, USA), Iron(II) chloride anhydrous(Alfa Aesar, USA), Hydrogen tetrachloroaurate(III) hydrate(Alfa Aesar, USA), (1-Hexadecyl) trimethylammonium bromide(Alfa Aesar, USA), Hydrazine hydrate(Acros Organics, USA), Poly(styrene-alt-maleic acid) sodium salt solution (PSMA)(Aldrich, USA), L-Ascorbic acid(Sigma-Aldrich, USA), Hydrogen peroxide solution(Sigma, USA), p-Toluenesulfonic Acid, 12% in Acetic Acid (TA) (Acros Organics, USA), N, N-Dimethyl-4-nitrosoaniline (RNO) (Alfa Aesar, USA).
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