Patient tumours were observed at × 100 magnification using a highly modified Olympus microscopy system. The microscope was attached to a cantilevered arm to extend over the patient. Given the high magnification required to observe individual vessels and flow, vibrations in the microscope were dampened using a weighted marble base of over 360 kg. Fluorescein was illuminated using a 467–498-nm excitation, 513–556 emission dichronic filter set (Spectra Services, Rochester, NY) with an X-Cite 120 Led light source (Lumen Dynamics, Ontario, Canada). Images were captured using a Luca EMCCD camera (Andor Technology Ltd., Belfast, Northern Ireland) controlled through the Solis acquisition and analysis programme. Images were acquired with a minimum of a 0.05-s exposure at 20 frames a second. Offline quantification of vessel diameter and blood velocity was performed using the ImageJ software suite.
X cite 120 led light source
Manufactured by Excelitas
Sourced in Canada
The X-Cite 120 LED light source is a compact, high-performance LED illumination system designed for use in a variety of laboratory applications. The device provides stable, uniform, and adjustable light output across a wide range of wavelengths, making it suitable for various microscopy, imaging, and spectroscopy techniques.
Automatically generated - may contain errors
Lab products found in correlation
Elements software, by Nikon (2 mentions)
Bx51 microscope, by Olympus (2 mentions)
Ds ri1, by Nikon (2 mentions)
Epifluorescent microscope 80i, by Nikon (2 mentions)
Luca emccd camera, by Oxford Instruments (1 mentions)
Amt image capture engine v602, by Advanced Microscopy Techniques (1 mentions)
1200 ex transmission, by JEOL (1 mentions)
Ti e inverted microscope, by Nikon (1 mentions)
Sola se light engine, by Lumencor (1 mentions)
Axiovert 200m microscope, by Zeiss (1 mentions)
Alexa fluor 647, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 568, by Thermo Fisher Scientific (1 mentions)
Csu x1, by PerkinElmer (1 mentions)
6 protocols using x cite 120 led light source
1
Intravital Microscopy of Tumor Vasculature
2
Microscopy Imaging of Bacterial Morphology
3
Fluorescence Imaging of Fixed Cells
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Phase contrast and fluorescence images of fixed cells were acquired from samples on 1% agarose/PBS pads with an Olympus BX51 microscope equipped with a 100X Plan N (N.A. = 1.25) Ph3 objective (Olympus), X-Cite 120 LED light source (Lumen Dynamics), and an OrcaERG CCD camera (Hammamatsu Photonics) or a Nikon TiE inverted microscope equipped with a 100X Plan N (N.A. = 1.25) objective (Nikon), SOLA SE Light Engine (Lumencor), heated control chamber (OKO Labs), and ORCA-Flash4.0 sCMOS camera (Hammamatsu Photonics). Filter sets for fluorescence were purchased from Chroma Technology Corporation. Nikon Elements software (Nikon Instruments) was used for image capture.
4
Fluorescent and Histochemical Imaging Protocol
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Histological examination of the slide-mounted tissue was examined using a Nikon epifluorescent microscope 80i (Nikon Instruments Inc., Melville, NY) and an X-Cite 120 LED light source (Excelitas Technologies®, Waltham, MA). The following sets of filters were used for visualization fluorescent labeling in conjunction with the histochemical or immunohistological labeling: TRITC excitation of 533–553 nm and an emission of 573–613 nm and FITC excitation of 464.5–499.5 and an emission of 516–556 nm. Combinations of these filters resulted in negligible cross-talk between the signals from individual fluorochromes, such that there was minimal to no observable bleed-through between any of the three signals. Visualization of DAB-labeled sections was done with incandescent illumination. All photomicrographs of the images were taken using a Nikon DS-Ri1 using the NIS software from Nikon.
5
Fluorescence Microscopy Imaging Protocol
6
Confocal Microscopy of MDCK Cells
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Confocal images were acquired at 37°C using a spinning disk confocal head (CSU-X1; PerkinElmer) coupled to a fully motorized inverted Axiovert 200-M microscope (ZEISS) equipped with Plan Apochromat; 63×/1.4 oil DIC objective and with a X-Cite 120LED light source (Excelitas) for wide-field illumination. Solid-state lasers (480, 568, and 660 nm) coupled to the spinning head through a fiber optic were used as a light source. An acoustic-optical tunable filter was used to switch between different wavelengths. The imaging system operates under control of SlideBook 6 (Intelligent Imaging Innovations, Inc.) and includes a computer-controlled spherical aberration correction device (SAC; Intelligent Imaging Innovations, Inc.) installed between the objective lens and the charge-coupled device camera (QuantEM:512SC; Photometrics). Postacquisition images were scaled linearly using ImageJ. For immunofluorescence, MDCK monolayers were fixed in 4% paraformaldehyde, washed, and stained for HA-FcRn-EGFP, E-cadherin, or GP135 as described in General cell health characterization. Secondary anti–mouse Fab2 labeled with Alexa Fluor 647 and anti–rat Fab2 labeled with Alexa Fluor 568 antibodies from Thermo Fisher Scientific were used.
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