After the achievement of informed consent from donors, non-carious, intact human third molars were gathered in accordance with a protocol approved by the Ethics Committee of Hospital of Stomatology, Wuhan University (certificate 2019A11). All molars were thoroughly cleaned, rinsed, and immersed in a 0.5% thymol solution at 4 °C prior to use. A water-cooled, low-speed Isomet diamond saw (Buehler, USA) was used to segment the molars perpendicular to the long axis beneath the enamel–dentinal junction to produce dentin disk specimens, and 600-, 800-, 1200-, 2000-, and 3000-grit silicon carbide sandpapers were employed to sequentially wet-polish the specimens to 1 mm in thickness. Sterile deionized water was used to thoroughly rinse the surface of each specimen.
Isomet diamond saw
Manufactured by Buehler
Sourced in United States
The Isomet diamond saw is a precision cutting instrument designed for accurate and efficient sectioning of a variety of materials. It features a diamond-coated blade that provides a clean, smooth cutting surface. The saw is suitable for use in a range of laboratory and research applications that require precise and controlled sample preparation.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using isomet diamond saw
1
Preparation of Dentin Disk Specimens
2
Preparing Dentin Disc Specimens for Permeability Study
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This study was conducted in accordance with the Declaration of Helsinki. After achieving donors’ informed consents according to the protocol approved by the Ethics Committee of School and Hospital of Stomatology, Wuhan University [no. 2019 (A11)], sound human third molars were obtained, cleaned, and reserved in thymol solution [0.5% (w/v)] at 4 °C. Dentin disc specimens (1.0 ± 0.1 mm in thickness, 6.0 ± 0.5 mm in diameter) were prepared using a water-cooling, low-speed Isomet diamond saw (Buehler, Lake Bluff, IL, USA) to slice molars below the enamel–dentinal junction, which was parallel to the occlusal plane. These discs were uniformly burnished to 1.0 mm thickness while excluding unqualified ones for minimizing the effect on measuring the dentin permeability.
3
Silver Nitrate Characterization by SEM
4
Push-out Bond Strength Evaluation of Dental Cements
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The teeth were embedded in acrylic blocks prepared as
apical thirds in acrylic. Parallel transverse sections were
obtained with a water-cooled low-speed IsoMet diamond
saw (Buehler, Lake Bluff, NY, USA) from the coronal to
the apical direction (three slices per tooth) (3 (link)). A total of
240 dentin slices (approximately 1 mm-thickness) were
obtained. The thickness of each slice was measured using
digital calipers (Teknikel, Istanbul, Turkey) with an accuracy
of 0.001 mm. The homogeneity of the groups in terms of
slice thickness was confirmed through analysis of variance
(ANOVA) (p>0.05).
A continuous load was applied to the center of the cements
tested using a stainless steel cylindrical plunger of one mm
in diameter, mounted onto a Lloyd LRX universal testing
machine (Lloyd Instruments, Ltd., Fareham, UK). Loading
was applied with a speed of 1 mm min−1 from the apical to
coronal direction until dislodgement of the cement occurred.
All three slices of each teeth were tested using the push-out
test and the mean was taken. The push-out bond strength was
calculated in megapascals (MPa) by dividing the maximum
load at failure (N) by the area of surface adhesion using the
formula (19 (link)), area = 2πr × h (where π = 3.14, a constant value,
r = radius of intraradicular space, and h = slice thickness in
mm) (20 (link)).
apical thirds in acrylic. Parallel transverse sections were
obtained with a water-cooled low-speed IsoMet diamond
saw (Buehler, Lake Bluff, NY, USA) from the coronal to
the apical direction (three slices per tooth) (3 (link)). A total of
240 dentin slices (approximately 1 mm-thickness) were
obtained. The thickness of each slice was measured using
digital calipers (Teknikel, Istanbul, Turkey) with an accuracy
of 0.001 mm. The homogeneity of the groups in terms of
slice thickness was confirmed through analysis of variance
(ANOVA) (p>0.05).
A continuous load was applied to the center of the cements
tested using a stainless steel cylindrical plunger of one mm
in diameter, mounted onto a Lloyd LRX universal testing
machine (Lloyd Instruments, Ltd., Fareham, UK). Loading
was applied with a speed of 1 mm min−1 from the apical to
coronal direction until dislodgement of the cement occurred.
All three slices of each teeth were tested using the push-out
test and the mean was taken. The push-out bond strength was
calculated in megapascals (MPa) by dividing the maximum
load at failure (N) by the area of surface adhesion using the
formula (19 (link)), area = 2πr × h (where π = 3.14, a constant value,
r = radius of intraradicular space, and h = slice thickness in
mm) (20 (link)).
5
In-vivo Bone Labeling Protocol
6
Microhardness Evaluation of Dental Samples
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All teeth were mesiodistally sectioned into equal cross-sectional parts using a water-cooled low-speed Isomet diamond saw (Buehler Ltd., Lake Bluff, IL, USA). The resulting teeth sections were positioned on self-cure acrylic-filled cylindrical molds, with the sectioned surface facing outward and then metallographically polished using a series of 400, 500, and 600 silicon carbide abrasive grit papers. The specimens were finely polished with a water-based diamond paste of 1 to 0.25 mm (Buehler, Lake Bluff, IL, USA) at low speed over a 180-s interval to provide a flat surface, which was confirmed at x40 magnification. This protocol was adopted because microhardness requires polished dental surfaces aiming to allow an adequated visualization of the indentations (Albuquerque et al., 2016) .
Knoop microhardness was evaluated using a microhardness tester (Shimadzu HMV-2000, Shimadzu Corporation, Kyoto, Japan). Settings for load and penetration were 25 g and 15 s, respectively. Knoop penetrations were made on the acrylic surface of each sample (apical, cervical, and middle root regions adopted in the present study) at depths of 0.01, 0.11, and 0.21 mm (Fig. 2A). Three measurements were performed for each distance, and a mean value was calculated.
Knoop microhardness was evaluated using a microhardness tester (Shimadzu HMV-2000, Shimadzu Corporation, Kyoto, Japan). Settings for load and penetration were 25 g and 15 s, respectively. Knoop penetrations were made on the acrylic surface of each sample (apical, cervical, and middle root regions adopted in the present study) at depths of 0.01, 0.11, and 0.21 mm (Fig. 2A). Three measurements were performed for each distance, and a mean value was calculated.
7
Tensile Bond Strength of Dental Adhesives
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After storage in 37°C water for 24 h, each bonded tooth was sectioned into beams (cross-sectional area approximately 1mm 2 ) using an Isomet diamond saw (Isomet 1000, Buehler, Lake Bluff, Illinois, USA). For each tooth (n = 5), three beams from the central area were randomly selected for µTBS, therefore resulting in a total of 15 beams to be tested. The remainder of the beams was stored for longer-term testing.
The beams were fixed to a Ciucchi's jig with cyanoacrylate glue (Model Repair 2 Blue, Dentsply-Sankin, Otahara, Japan) and subjected to a tensile force at a crosshead speed of 1 mm/min in a desktop testing apparatus (EZ test, Shimadzu, Kyoto, Japan). µTBS was expressed in MPa, and data were analyzed by three-way ANOVA and Dunnett T3 tests (α=0.05).
The beams were fixed to a Ciucchi's jig with cyanoacrylate glue (Model Repair 2 Blue, Dentsply-Sankin, Otahara, Japan) and subjected to a tensile force at a crosshead speed of 1 mm/min in a desktop testing apparatus (EZ test, Shimadzu, Kyoto, Japan). µTBS was expressed in MPa, and data were analyzed by three-way ANOVA and Dunnett T3 tests (α=0.05).
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