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Bio safe 2 liquid scintillation mixture

Manufactured by Research Products International
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Sourced in United States, Malta, Israel

Bio-Safe II is a liquid scintillation mixture formulated for counting radioactive samples. It is designed to provide efficient and reliable performance in liquid scintillation counting applications.

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Lab products found in correlation

3h cholecystokinin 8 cck 8, by PerkinElmer (3 mentions) Triton x 100, by Merck Group (3 mentions) Antibiotic antimycotic solution, by Merck Group (3 mentions) Fetal bovine serum (fbs), by Cytiva (3 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) 3h estradiol 17 β d glucuronide e217βg, by PerkinElmer (2 mentions) E217βg, by Merck Group (2 mentions) Trypsin edta solution, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) 3h e217βg, by PerkinElmer (2 mentions) Dulbecco s modified eagle medium dmem, by Merck Group (2 mentions) Rifampicin, by Merck Group (2 mentions) Dasatinib, by LC Laboratories (2 mentions) Unlabeled cck 8, by Merck Group (2 mentions) Nicardipine, by Santa Cruz Biotechnology (1 mentions) Hank s balanced salt solution hbss, by Merck Group (1 mentions) Dulbecco s modified eagle medium, by Merck Group (1 mentions) Hepes, by BD (1 mentions) Geneticin, by BD (1 mentions) Insulin transferrin selenium, by BD (1 mentions)

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Bio-Safe II (7 citations)

5 protocols using bio safe 2 liquid scintillation mixture

1

Receptor Binding Assay for E2 and CCK-8

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[3H]-estradiol 17 β-D-glucuronide (E217βG) (specific activity 37.2 Ci/mmol) and [3H]-cholecystokinin-8 (CCK-8) (specific activity 98.4 Ci/mmol) were purchased from Perkin Elmer Life Science (Waltham, MA, USA). Nicardipine was purchased from Santa Cruz Biotechnology (Dallas, TX, USA) Unlabeled CCK-8, E217βG, dimethyl sulfoxide (DMSO), Dulbecco’s Modified Eagle Medium (DMEM), Hank’s Balanced Salt Solution (HBSS), antibiotic antimycotic solution, trypsin–EDTA solution, Triton X-100, and fetal bovine serum (FBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Poly-L-lysine was purchased from Trevigen Inc. (Gaithersburg, MD, USA). Geneticin® and HEPES were purchased from BD Biosciences (Bedford, MA, USA). Bio-Safe II liquid scintillation mixture was purchased from Research Products International (Mt. Prospect, IL, USA).
Kayesh R., Tambe V., Xu C, & Yue W. (2023). Differential Preincubation Effects of Nicardipine on OATP1B1- and OATP1B3-Mediated Transport in the Presence and Absence of Protein: Implications in Assessing OATP1B1- and OATP1B3-Mediated Drug–Drug Interactions. Pharmaceutics, 15(3), 1020.
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2

Radioligand Binding Assay Protocol

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[3H]-E217βG (specific activity 41.4 Ci/mmol) and [3H]-E1S (specific activity 54.0 Ci/mmol) were purchased from Perkin Elmer Life Science (Waltham, MA). Dasatinib and CsA were purchased from LC laboratories (Woburn, MA, USA). Rifampicin, unlabeled E217βG, Hanks’ balanced salt solution (HBSS), dimethyl sulfoxide (DMSO), Triton X-100, Dulbecco’s Modified Eagle Medium (DMEM), Dulbecco’s phosphate buffered saline (DPBS), trypsin-EDTA solution, and antibiotic antimycotic solution were purchased from Sigma-Aldrich (St. Louis, MO). Poly-L-lysine was purchased from Trevigen Inc. (Gaithersburg, MD). Geneticin® (G418), BioCoat™ culture plates, insulin/transferrin/selenium (ITS+), and Matrigel™ were purchased from BD Biosciences (Bedford, MA). Fetal bovine serum (FBS) was purchased from Hyclone Laboratories (Logan, Utah). Bio-Safe II liquid scintillation mixture was purchased from Research Products International (Mt. Prospect, IL).
Pahwa S., Alam K., Crowe A., Farasyn T., Neuhoff S., Hatley O., Ding K, & Yue W. (2017). Pretreatment with Rifampicin and Tyrosine Kinase Inhibitor Dasatinib Potentiates the Inhibitory Effects toward OATP1B1- and OATP1B3-Mediated Transport without Affecting Plasma Membrane Localization of the Transporters. Journal of pharmaceutical sciences, 106(8), 2123-2135.
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3

Radioligand Binding Assay for CCK-8 and E2 17βG

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[3H]-Cholecystokinin 8 (CCK-8) (specific activity 88.0 Ci/mmol) and [3H]-estradiol 17 β-D-glucuronide (E217βG) (specific activity 41.4 Ci/mmol) were purchased from Perkin Elmer Life Science (Waltham, MA). Vemurafenib was purchased from LC laboratories (Woburn, MA, USA). Unlabeled CCK-8, E217βG, Triton X-100, dimethyl sulfoxide (DMSO), Hank’s Balanced Salt Solution (HBSS), Dulbecco’s Modified Eagle Medium (DMEM), trypsin-EDTA solution, and antibiotic antimycotic solution were purchased from Sigma-Aldrich (St. Louis, MO). Poly-L-lysine was purchased from Trevigen Inc. (Gaithersburg, MD). Geneticin® and HEPES was purchased from BD Biosciences (Bedford, MA). Fetal bovine serum (FBS) was purchased from Hyclone Laboratories (Logan, Utah). Bio-Safe II liquid scintillation mixture was purchased from Research Products International (Mt. Prospect, IL). Human plasma was purchased from BioIVT (Hicksville, NY, Lot#BRH895613).
Kayesh R., Farasyn T., Crowe A., Liu Q., Pahwa S., Alam K., Neuhoff S., Hatley O., Ding K, & Yue W. (2020). Assessing OATP1B1- and OATP1B3-mediated drug-drug interaction potential of vemurafenib using R-value and physiologically based pharmacokinetic models. Journal of pharmaceutical sciences, 110(1), 314-324.
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4

Radioactive Labeling and Inhibitor Protocol

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[3H]E217βG (specific activity 41.4 Ci/mmol) and 32P-orthophosphate (10 mCi) were purchased from PerkinElmer Life Science (Waltham, MA). Complete protease inhibitor cocktail tablets, Phosstop phosphatase inhibitor tablets were purchased from Roche Diagnostics (Indianapolis, IN). Geneticin (G418) and EZ-Link™ Sulfo-NHS-SS-Biotin were purchased from Thermo Fisher Scientific (Carlsbad, CA). Protein A/G beads were purchased from Santa Cruz Biotechnology (Dallas, TX). Unlabeled estradiol 17β-glucuronide (E217βG), lactic acid dehydrogenase (LDH) cytotoxicity kit and fetal bovine serum (FBS) were purchased from Sigma Aldrich (St. Louis, MO). Bio-Safe II liquid scintillation mixture and 1,4-dithiothreitol (DTT) were purchased from Research Products International (Mt. Prospect, IL). Other reagents, unless specified, were purchased from Sigma Aldrich (St. Louis, MO), Thermo Fisher Scientific (Carlsbad, CA), or VWR International (Radnor, PA).
Crowe A., Zheng W., Miller J., Pahwa S., Alam K., Fung K.M., Rubin E., Yin F., Ding K, & Yue W. (2019). Characterization of plasma membrane localization and phosphorylation status of organic anion transporting polypeptide (OATP) 1B1 c.521 T>C nonsynonymous single-nucleotide polymorphism. Pharmaceutical research, 36(7), 101.
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5

Characterization of Radiolabeled Compounds

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[ 3 H]cholecystokinin 8 (CCK-8) (specific activity 98.7.0 Ci/mmol) was purchased from Perkin Elmer Life Science (Waltham, MA). [ 3 H]Pitavastatin (specific activity 5 Ci/mmol), unlabeled pitavastatin and [ 3 H]rosuvastatin (specific activity 20 Ci/mmol) were purchased from American Radiolabeled Chemicals (St. Louis, MO). Unlabeled rosuvastatin was purchased from Toronto Research Chemicals, Inc. (North York, ON, Canada). EVR, SIR, and dasatinib were purchased from LC laboratories (Woburn, MA). Unlabeled CCK-8, bromosulfophtalein (BSP), rifampicin, and Dulbecco's Modified Eagle Medium (DMEM) were purchased from Sigma-Aldrich (St. Louis, MO). Fetal bovine serum (FBS) was purchased from Hyclone Laboratories (Logan, Utah). Bio-Safe II liquid scintillation mixture was purchased from Research Products International (Mt. Prospect, IL). All other materials were purchased from Thermo Fisher Scientific (Waltham, MA), Sigma-Aldrich (St. Louis, MO), or VWR International (Radnor, PA).
Farasyn T., Pahwa S., Xu C, & Yue W. (2021). Pre-incubation with OATP1B1 and OATP1B3 inhibitors potentiates inhibitory effects in physiologically relevant sandwich-cultured primary human hepatocytes. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 165.
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