Compritol 888

Stearic acid and SPAN 80 were purchased from Merck (Merck KGaA, Darmstadt, Germany). Arachidic acid, Tween 60, Tween 80, poly(vinyl alcohol), L-lysine monohydrochloride, lithium carbonate, dansyl chloride, methylamine hydrochloride, triethylamine and sodium acetate were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Miglyol 812 was purchased from Caelo (Caesar & Loretz GmbH, Hilden, Germany). Precirol ATO 5 and Compritol 888 ATO were kindly provided by Gattefossé (Saint Priest Cedex, France). L-Phenylalanine ethyl-ester hydrochloride was purchased from Fluka (Fluka Chemie GmbH, Buchs, Switzerland), acetic acid was obtained from VWR Chemicals (VWR International S.A.S., Fontenay-sous-Bois, France) and acetonitrile and methanol were obtained from Honeywell (Honeywell Riedel-de Häen AG, Seelze, Germany). Aqueous solutions were prepared with double-deionized water (Arium Pro, Sartorius AG, Göttingen, Germany).

The morin hydrate (MH), egg yolk L-α-phosphatidylcholine (PC) (lecithin), and triolein were purchased from Sigma-Aldrich, Inc., St. Louis, MO, USA. The Brij52 (polyoxyethylene (2) cetyl ether) was obtained from BASF Co. (Florham Park, NJ, USA). The compritol 888 (glyceryl behenate) was acquired as gift from Gattefosse (Saint-Priest, France). The sodium chloride, potassium dihydrogen orthophosphate, methanol, sodium hydroxide, magnesium chloride, chloroform, and absolute ethanol were purchased from El-Gomhouria Chemical Co., Cairo, Egypt. The dialysis membranes (Spectra/Pore^®, cut-off 12,000–14,000) were purchased from Spectrum Laboratories Inc., Rancho Dominguez, CA, USA). All chemicals and solvents were of analytical grade and were used as received. The specific ELISA kits were obtained from eBioscience Co, San Diego, CA, USA. The Vero cells (African green monkey kidney cells, Vero C1008 (Vero 76, clone E6, Vero E6)) were purchased from Viromed Laboratories (Minnetonka, MN, USA).