Fresh tissue was placed in fixative containing 2% paraformaldehyde and 3% gluteraldehyde in pH 7.4 phosphate buffer overnight at 4°C. Tissue was washed once in PBS, followed by post-fixation with 1% osmium tetroxide. Samples were dehydrated in washes with ascending concentrations of alcohol, followed by embedding in Araldite. Sections were cut and stained with uranyl acetate and lead citrate. Imaging was performed on Olympus EM208S transmission electron microscope.
Em208s transmission electron microscope
Manufactured by Olympus
Sourced in Japan
The EM208S is a transmission electron microscope (TEM) manufactured by Olympus. It is a scientific instrument used for high-resolution imaging and analysis of samples at the nanoscale level. The EM208S enables visualization and characterization of the internal structure and composition of materials, making it a valuable tool for a variety of applications in fields such as materials science, nanotechnology, and biological research.
Automatically generated - may contain errors
9 protocols using em208s transmission electron microscope
1
Tissue Preparation for TEM Imaging
2
Ultrastructural analysis of Giardia trophozoites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
G. duodenalis trophozoites were fixed in 1% glutaraldehyde and 4% paraformaldehyde in 0.1 M sodium cacodylate buffer, pH 7.2, overnight, at 4 °C, and processed according to Perry and Gilbert with slight modifications [42 (link)]. Parasites were washed in cacodylate buffer and post-fixed with 1% OsO4 in 0.1 M sodium cacodylate buffer for 1 h, at RT, treated with 1% tannic acid in 0.05 M cacodylate buffer for 30 min and rinsed in 1% sodium sulfate in 0.05 M cacodylate buffer for 10 min. Post-fixed specimens were washed, dehydrated through a graded series of ethanol solutions (30–100% ethanol) and embedded in Agar 100 (Agar Scientific Ltd., Stansted, Essex, UK). Ultrathin sections, obtained by an UC6 ultramicrotome (Leica Microsystems, Wetzlar, Germany), were stained with uranyl acetate and Reynolds’ lead citrate and examined at 100 kV with a FEI/Philips EM 208S Transmission Electron Microscope equipped with acquisition system/Megaview SIS camera (Olympus, Hamburg, Germany).
3
Transmission Electron Microscopy of HSC Ferroptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
According to our previous reports [12 (link)], morphological characteristics of HSC ferroptosis was performed by transmission electron microscopy assay. In brief, HSC-LX2 cells (20,000 cells/well) were cultured and inoculated onto 4-well Chambered Coverglass (155382, Thermo Fisher Scientific). Olympus EM208S transmission electron microscope was used to acquire the images.
4
Ultrastructural Analysis of Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were seeded onto 4-chambered coverglass (Lab-tek Chambered Coverglass System, Nalgene-Nunc, Rochester, NY, USA) at a density of 2X104 cells/ml (14,000 cells/well). After treatment cells were fixed with 2.5% glutaraldehyde and washed 3 times with PBS. Subsequent post-fixation with 1% osmium tetroxide followed by dehydration with an ascending series of alcohol before embedding samples in araldite (SIGMA-ALDRICH, A3183). Ultrathin sections were cut and doubly stained with uranyl acetate and lead citrate. Images were acquired using the Olympus EM208S transmission electron microscope.
5
Ultrastructural Analysis of Treated MSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After starvation or combined treatment of TNF‐α, IFN‐γ, and TGF‐β1, MSCs were rinsed with PBS and fixed with 2% glutaraldehyde (cat. no. G5882; Sigma) in 0.1 M phosphate buffer (pH 7.2) for 2 hr at 4°C. The cells were postfixed with 1% osmic acid for another 2 hr at 4°C, followed by dehydration using ethanol in graded concentrations. Subsequently, the sections were incubated with uranylacetate and lead citrate (cat. no. 15326; Sigma), and images were acquired using an Olympus EM208S transmission electron microscope (Olympus, Tokyo, Japan).
6
Ultrastructural Analysis of Mouse Hepatocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary mouse hepatocytes were seeded onto four-chambered coverglass (Lab-Tek Chambered Coverglass System; Nalgene-Nunc, Rochester, NY) at a density of 2 × 104 cells/ml. Cells were fixed with 2.5% glutaraldehyde in 0.1 M sodium cacodylate and 1 mM CaCl2 (pH 7.4) and postfixed in 1% osmium tetroxide (OsO4). Then, the samples were dehydrated in graded ethanol, from 50% to 100%, and embedded in Spurr’s resin. Ultrathin sections (90 nm) were cut and placed on 150 mesh copper grids. After staining with lead citrate and uranyl acetate (2% in 50% ethanol), images were acquired using an Olympus EM208S transmission electron microscope (Olympus, Tokyo, Japan).
7
Transmission Electron Microscopy Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transmission electron microscopy was performed following previously described methods.8 (link)–10 (link) Briefly, cells were seeded onto 4-well Chambered Coverglass at a density of 2 × 104 cells/mL. The cells were fixed with 2.5% glutaraldehyde and 2% paraformaldehyde in 0.1 M phosphate buffer at 4°C for 2 hours. Postfixation was carried out with 1% osmium tetroxide in the same buffer for 1 hour at room temperature, followed by dehydration in a graded series of ethanol and embedding in Epon resin. Ultrathin sections were cut with an ultramicrotome and examined using an Olympus EM208S transmission electron microscope for image acquisition.
8
Tissue and Cell Fixation for TEM Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fresh tissue was placed in fixative (2% para-formaldehyde and 3% gluteraldehyde in pH 7.4 cacydolate buffer) and stored at 4 °C, whereas cultured cells were grown in glass chambers, and fixed overnight in fixative (2% para-formaldehyde and 3% gluteraldehyde in pH 7.4 phosphate buffer). Samples were washed once in PBS, followed by post-fixation treatment with 1% osmium tetroxide. Samples were dehydrated with ascending concentrations of alcohol, followed by Araldite embedding. Ultrathin sections were cut then stained with uranyl acetate and lead citrate. Images were acquired on an Olympus EM208S transmission electron microscope.
9
Ultrastructural Analysis of Autophagy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fresh tissue was placed in fixative containing 2% paraformaldehyde and 3% gluteraldehyde in pH 7.4 phosphate buffer overnight at 4°C. Tissue was washed once in PBS, followed by post-fixation with 1% osmium tetroxide. Samples were dehydrated in washes with ascending concentrations of alcohol, followed by embedding in Araldite. Ultra-thin sections were cut and stained with uranyl acetate and lead citrate. Imaging was performed on an Olympus EM208S transmission electron microscope. Autophagic vesicles were defined as autophagosomes (double-membraned structures surrounding cytoplasmic material) and autolysosomes (lysosomes containing cytoplasmic material). Vesicles were counted in 10 random fields per mouse, in 3 mice per condition.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!