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Pcr focused immunopathology microrna array plates

Manufactured by Qiagen
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The PCR focused immunopathology microRNA array plates are a specialized laboratory equipment designed for the analysis of microRNA expression profiles in immunological and pathological samples. These plates provide a comprehensive platform for the simultaneous detection and quantification of multiple microRNA targets in a single experiment.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (2 mentions) Rt2 microrna first strand kit, by Qiagen (2 mentions) Cfx96 connect real time pcr system, by Bio-Rad (1 mentions)

2 protocols using pcr focused immunopathology microrna array plates

1

Inflammatory microRNA Expression in Macrophages

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To determine the expression of inflammatory microRNAs, WT and 5-LO−/− macrophages were treated with or without LTB4 for 24 h. RNA was then extracted from the cells using a Qiagen RNeasy mini kit according to the manufacturer’s instructions. cDNA was synthesized using the RT2 microRNA First Strand Kit (Qiagen) and applied to PCR focused immunopathology microRNA array plates (Qiagen). Plates were processed in a BioRad CFX96 Connect Real-Time PCR System using automated baseline and threshold cycle detection.
Wang Z., Filgueiras L., Wang S., Serezani A.P., Peters-Golden M., Jancar S, & Serezani C.H. (2014). Leukotriene B4 enhances the generation of pro-inflammatory microRNAs to promote MyD88-dependent macrophage activation. Journal of immunology (Baltimore, Md. : 1950), 192(5), 2349-2356.
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2

Profiling Inflammatory microRNAs in Macrophages

Cited 1 time
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To determine the expression of inflammatory microRNAs, macrophages from C57BL/6 WT mice were treated with PTEN siRNA or scrambled siRNA as above. RNA was then extracted from the cells using Qiagen RNeasy mini kit according to the manufacturer’s instructions. cDNA was synthesized using the RT2 microRNA First Strand Kit (Qiagen) and applied to PCR focused immunopathology microRNA array plates (Qiagen) as previously shown (10 (link)). Normalization and statistical analysis of miRNA expression was conducted using SABiosciences’ Online PCR Array Data Analysis Web Portal as previously described (10 (link)).
Sisti F., Wang S., Brandt S.L., Glosson-Byers N., Mayo L., Son Y.M., Sturgeon S., Filgueiras L., Jancar S., Wong H., Dela Cruz C.S., Andrews N., Alves-Filho J.C., Cunha F.Q, & Serezani C.H. (2018). Nuclear PTEN enhances the maturation of a microRNA regulon to limit MyD88-dependent susceptibility to sepsis. Science signaling, 11(528), eaai9085.
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