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Goat anti mouse or anti rabbit igg peroxidase conjugated secondary antibodies

Manufactured by Merck Group
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Goat anti-mouse or anti-rabbit IgG peroxidase-conjugated secondary antibodies are laboratory reagents used to detect and quantify the presence of mouse or rabbit primary antibodies in various immunoassays and immunostaining techniques. These secondary antibodies are conjugated with the enzyme horseradish peroxidase, which can catalyze a colorimetric or chemiluminescent reaction for signal detection.

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Lab products found in correlation

Sulforhodamine b srb, by Merck Group (4 mentions) Dimethyl sulfoxide (dmso), by Merck Group (3 mentions) Hoechst 33342, by Merck Group (2 mentions) P53 do 1, by Santa Cruz Biotechnology (2 mentions) Anti actin, by Merck Group (2 mentions) Anti caspase 9, by Cell Signaling Technology (2 mentions) Rabbit polyclonal antibody against actin, by Merck Group (1 mentions) Gant61, by Merck Group (1 mentions) Antibodies against akt and phospho akt ser473, by Cell Signaling Technology (1 mentions) Antibodies against beclin1, by Abcam (1 mentions) Anti cleaved caspase 3 asp175, by Cell Signaling Technology (1 mentions) Antibody against lc3, by Novus Biologicals (1 mentions) Anti caspase 8 1c12, by Cell Signaling Technology (1 mentions) Anti caspase 8, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Goat anti-mouse or -rabbit IgG peroxidase conjugated secondary antibodies Peroxidase-conjugated anti-rabbit or anti-mouse secondary antibodies Peroxidase-conjugated goat anti-mouse/anti-rabbit IgG antibodies Anti-mouse or anti-rabbit secondary antibodies Goat anti-mouse peroxidase-conjugated antibodies Peroxidase-conjugated-anti-rabbit antibodies Anti-mouse secondary antibodies Goat anti-rabbit antibodies Mouse secondary antibodies Rabbit IgG antibodies

4 protocols using goat anti mouse or anti rabbit igg peroxidase conjugated secondary antibodies

1

Molecular Mechanisms in Cancer Research

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DMSO, GANT-61, Sulforhodamine B (SRB) and Hoechst 33342 were purchased from Sigma Aldrich (Milano, Italy). GDC-0449 (CUR-0199691) was provided by Genentech (San Francisco, CA). Antibodies against AKT, and phospho-AKT were obtained from Cell Signaling Technology (MA, USA). Antibodies against Bax and Bcl-2 were from BD Pharmigen (BD Biosciences, San Josè, CA, USA). Antibodies against GLI1 (H-300), Shh (H-160), ERK1/2 (C-14), phospho-ERK (E-4), NF-κB (p65), PARP-1, p53 (DO-1) and H-Ras (259) were obtained from Santa Cruz Biotechnology (CA, USA). Anti-ErbB2 and anti-EGFR antisera were provided by Dr. M.H. Kraus (University of Alabama, Birmingham, USA). Goat anti-rabbit IgG Alexa fluor-594-conjugated and goat anti-mouse IgG Alexa fluor-488-conjugated secondary antibody came from Invitrogen (Milano, Italy). A rabbit polyclonal antibody against actin and goat anti-mouse or anti-rabbit IgG peroxidase-conjugated secondary antibodies were from Sigma-Aldrich.
Benvenuto M., Masuelli L., De Smaele E., Fantini M., Mattera R., Cucchi D., Bonanno E., Di Stefano E., Frajese G.V., Orlandi A., Screpanti I., Gulino A., Modesti A, & Bei R. (2016). In vitro and in vivo inhibition of breast cancer cell growth by targeting the Hedgehog/GLI pathway with SMO (GDC-0449) or GLI (GANT-61) inhibitors. Oncotarget, 7(8), 9250-9270.
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2

Immunoblotting Analysis of Signaling Proteins

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Sulforhodamine B (SRB) was purchased from Sigma-Aldrich (Saint Louis, MO, USA). Antibodies against AKT and phospho-AKT (Ser473) were obtained from Cell Signaling Technology (MA, USA). Antibodies against Bax and Bcl-2 were from BD Pharmigen (BD Biosciences, San Josè, CA, USA). Antibodies against ERK1/2 (C-14), phospho-ERK (E-4), PARP-1, and p53 (DO-1) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-ErbB2 antiserum was provided by Dr. M.H. Kraus (University of Alabama, Birmingham, USA). A rabbit polyclonal antibody against actin and goat anti-mouse or anti-rabbit IgG peroxidase-conjugated secondary antibodies were from Sigma-Aldrich.
Frajese G.V., Benvenuto M., Mattera R., Giampaoli S., Ambrosin E., Bernardini R., Giganti M.G., Albonici L., Dus I., Manzari V., Modesti A., Mattei M, & Bei R. (2018). Electrochemically Reduced Water Delays Mammary Tumors Growth in Mice and Inhibits Breast Cancer Cells Survival In Vitro. Evidence-based Complementary and Alternative Medicine : eCAM, 2018, 4753507.
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3

Molecular Signaling Pathway Investigation

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DMSO, (±)-Gossypol (GOS), and Sulforhodamine B (SRB) were purchased from Sigma Aldrich (Milano, Italy). Antibodies against AKT, phospho-AKT, Bax, Bcl-2, JNK/SAPK1, JNK/SAPK (pT183/pY185), p38a/ SAPK2a, p38 MAPK (pT180/pY182) were obtained from BD Pharmingen (BD Biosciences, San Jose, CA). Antibodies against ERK1/2 (C-14), phospho-ERK (E-4), PARP-1, and p53 (DO-1) were obtained from Santa Cruz Biotechnology (Dallas, TX). Antibody against LC3 was obtained from Novus Biologicals (Littleton, CO). Antibodies against Beclin-1 were obtained from Abcam (Cambridge, UK). The anti-caspase 9 (C9), anti-caspase 8 (1C12), and anti-cleaved caspase 3 (Asp175) antibodies were purchased from Cell Signaling Technology (Danvers, MA). Anti-ErbB2 and anti-EGFR antisera were provided by Dr. M.H. Kraus (University of Alabama, Birmingham, AL). Rabbit polyclonal anti-Actin was obtained from Sigma-Aldrich. Goat anti-mouse or anti-rabbit IgG peroxidase-conjugated secondary antibodies were purchased from Sigma-Aldrich.
Benvenuto M., Mattera R., Masuelli L., Taffera G., Andracchio O., Tresoldi I., Lido P., Giganti M.G., Godos J., Modesti A, & Bei R. (2017). (±)-Gossypol induces apoptosis and autophagy in head and neck carcinoma cell lines and inhibits the growth of transplanted salivary gland cancer cells in BALB/c mice. International journal of food sciences and nutrition, 68(3).
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4

Apoptosis Pathway Proteomics Analysis

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Reagents DMSO, sulforhodamine B (SRB), and Hoechst 33342 were purchased from Sigma-Aldrich (Milan, Italy). Rabbit polyclonal anti-Bax and mouse monoclonal anti-Bcl-2 antibodies were obtained from BD Pharmingen (BD Biosciences). Antibodies against ERK1/2 (C-14), phospho-ERK (E-4), NF-κB, PARP-1, and p53 (DO-1) were obtained from Santa Cruz Biotechnology (CA, USA). Antibody against light chain 3 (LC3) was obtained from Novus Biologicals (Littleton, CO, USA). Rabbit polyclonal anti-actin was from Sigma-Aldrich. The anti-caspase-9 and anti-caspase-8 antibodies were purchased from Cell Signaling Technology (MA, USA). Goat anti-mouse IgG Alexa Fluor-488-conjugated antibody was purchased from Life Technologies ™ Molecular Probes (Oregon, USA), and the goat anti-mouse or anti-rabbit IgG peroxidase-conjugated secondary antibodies were from Sigma-Aldrich.
Masuelli L., Pantanella F., La Regina G., Benvenuto M., Fantini M., Mattera R., Di Stefano E., Mattei M., Silvestri R., Schippa S., Manzari V., Modesti A, & Bei R. (2016). Violacein, an indole-derived purple-colored natural pigment produced by Janthinobacterium lividum, inhibits the growth of head and neck carcinoma cell lines both in vitro and in vivo. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 37(3).
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