Rose bengal sodium salt rb

Rose Bengal sodium salt (RB, MW: 1017.64 g/mol, ≥85%, Sigma, Madrid, Spain), tetrabutylammonium iodide (TBAI, MW: 369.37 g/mol, 98%, Aldrich Chemistry, Madrid, Spain), tetrabutylammonium chloride (TBACl, MW: 277.92 g/mol, ≥97%, Aldrich Chemistry, Madrid, Spain), Sodium iodide (NaI, Fisher Chemical, Madrid, Spain), 2-hydroxyethyl methacrylate (HEMA, MW: 130.14 g/mol, 97%, Acros Organics, Madrid, Spain), poly(ethylene glycol) dimethacrylate average Mn 550 (PEGDMA, Aldrich Chemistry, Madrid, Spain), 2,2′-azo-bis-isobutironitrile (AIBN, 98%, Merck, Madrid, Spain), 9,10-anthracenediyl-bis(methylene) dimalonic acid (ABDA, ≥90% (HPLC)), sodium phosphate monobasic dihydrate (≥99%, Sigma-Aldrich, Madrid, Spain), sodium phosphate dibasic anhydrous (≥99%, Fluka, Madrid, Spain), catalase from bovine liver (aqueous suspension, 10.000–40.000 units/mg protein, Sigma-Aldrich, Madrid, Spain), ammonium iron (II) sulfate hexahydrate (for analysis, Thermo Scientific, Madrid, Spain), xylenol orange sodium salt (Thermo Scientific, Madrid, Spain), sorbitol (≥97%, Fisher, Madrid, Spain), sulphuric acid (96%, Merck, Madrid, Spain) and hydrogen peroxide (for analysis, 35 wt.% solution in water, stabilized, Thermo Scientific, Madrid, Spain). All reagents were used as received.

Poly-ε-caprolactone (PCL, average Mn 80,000 g/mol) and polyvinyl alcohol (PVA, Mn = 30,000–70,000 g/mol, 87–90% hydrolyzed) were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Rose bengal sodium salt (RB) and dichloromethane (CH₂Cl₂) were acquired from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline (PBS, NaCl 0.138 M; KCl 0.0027 M; pH 7.4; 25 °C) and hydrochloric acid 37% (HCl, EMSURE^® ACS, ISO, Reag. Ph. Eur. analytical reagent, Supelco^®) were purchased from Sigma-Aldrich (Milan, Italy). Ultrapure bi-distilled water (H₂O) was obtained by a MilliQ R4 system, Millipore (Milan, Italy). Commercially pure titanium (c.p. Ti) grade IV was supplied by SEJOING Materials Co. Ltd. (Seoul, Korea) with mean particle size d[50] = 23.3 µm [28 (link)]. Ammonium bicarbonate (NH₄HCO₄) (BA), 99% purity, supplied by Cymit Química S.L. (Barcelona, Spain), 250–355 µm in size, was used as spacer particles [29 (link),30 (link)]. For the preparation of the solutions required by the titration method for the evaluation of bioactivity, sodium chloride (NaCl, BioXtra) ≥ 99.5% purity, di-sodium hydrogen phosphate dehydrate (Na₂HPO₄⋅2H₂O, EMPROVE^® ESSENTIAL Ph Eur, BP, USP), calcium chloride (CaCl₂) 97.0% purity, and Trizma base 99% purity (TRIS) were purchased by Sigma-Aldrich (Merck KGaA, Darmstadt, Germany).