6 diamidino 2 phenylindole dapi

Manufactured by RiboBio

Sourced in Germany

6-diamidino-2-phenylindole (DAPI) is a fluorescent stain that binds strongly to adenine-thymine (A-T) rich regions in DNA. It is commonly used in fluorescence microscopy to visualize and identify cell nuclei.

Automatically generated - may contain errors

Lab products found in correlation

Rna fish kit, by RiboBio (2 mentions) Laser scanning confocal microscope, by RiboBio (2 mentions) Rna fish probe mix, by RiboBio (1 mentions) Cyanine3 (cy3), by RiboBio (1 mentions)

2 protocols using 6 diamidino 2 phenylindole dapi

RNA FISH Localization Protocol

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RNA FISH Kit was from RiboBio (Guangzhou, China). The RNA FISH probe mix for LOC653786, 18S or U6 was synthesized and labeled with Cy3 by RiboBio. RNA FISH was performed as previously described [46 ]. The 6-diamidino-2-phenylindole (DAPI, RiboBio) was used for nuclei counterstaining, and the high resolution images were captured with a laser scanning confocal microscope (ZEISS, Jena, Germany).

Yang F., Wu Q., Zhang Y., Xiong H., Li X., Li B., Xie W., Zhang L., Xu M., Zhang K, & He F. (2018). LncRNA LOC653786 promotes growth of RCC cells via upregulating FOXM1. Oncotarget, 9(15), 12101-12111.

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RNA FISH Probe Synthesis and Imaging

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The RNA FISH probe mixture of KCNQ1DN, 18S or U6 RNA was synthesized and labeled with Cy3 from RiboBio. RNA FISH kit was purchased from RiboBio (Guangzhou, China). RNA FISH was performed as previously described15 . The 6-diamidino- 2-phenylindole (DAPI, RiboBio) was used for nuclei counterstaining, and high resolution images were taken using a laser scanning confocal microscope (ZEISS, Jena, Germany).

Yang F., Wu Q., Zhang L., Xie W., Sun X., Zhang Y., Wang L., Dai Q., Yu H., Chen Q., Sheng H., Qiu J., He X., Miao H., He F, & Zhang K. (2019). The long noncoding RNA KCNQ1DN suppresses the survival of renal cell carcinoma cells through downregulating c-Myc. Journal of Cancer, 10(19), 4662-4670.

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