GAPDH rabbit mAb (#2118), caspase-3 rabbit mAb (#9665), p-GSK3β (Ser9) rabbit antibody (#5558), GSK3β rabbit antibody (#9315), p-AKT (Thr308) rabbit antibody (#9275), p-AKT (Ser473) rabbit antibody (#4060), AKT rabbit antibody (#9272), Cleaved caspase 3 rabbit antibody (#9664) and caspase 3 rabbit antibody (#9662) were all obtained from Cell Signaling (Beverly, MA).
nCDase antibody (Ab174) was a kind gift from Dr. Rick Proia (Genetics of Development and Disease, NIDDK, Bethesda, MD) 11 (link). The enhanced chemiluminescence kit (ECL) was from ThermoScientific (Rockford, IL). C6 urea-ceramide was provided by the Lipidomics Core facility at MUSC.