HaCaT cell line purchased from China Infrastructure of Cell Line Resources (Beijing, China) was maintained in MEM/EBSS (HyClone, Logan, UT, USA) with 10 % FBS (VACCA Biologics, Green Bay, WI, USA) and 1 % penicillin/streptomycin (HyClone, Logan, UT, USA). Cells were cultured at 37˚C in a humid incubator with 5% CO2. HaCaT cells in logarithmic phase were treated with 100 ng/ml recombinant human IL-36γ (R&D Systems, Minneapolis, MN, USA), as follow Wnt signaling pathway pharmacological inhibitor IWP-2 (Sigma, St. Louis, MO, USA) 1 μM for 30 min.
Recombinant human il 36γ
Manufactured by R&D Systems
Sourced in United States
Recombinant human IL-36γ is a protein that is produced in a laboratory setting. It is a member of the interleukin-1 cytokine family and is involved in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Iwp 2, by Merck Group (1 mentions)
Vemurafenib, by Apexbio (1 mentions)
Selumetinib, by Selleck Chemicals (1 mentions)
Cetuximab, by Merck Group (1 mentions)
Anti erk, by Cell Signaling Technology (1 mentions)
Anti t7 antibody, by Abcam (1 mentions)
Anti phospho erk, by Cell Signaling Technology (1 mentions)
Trametinib, by Apexbio (1 mentions)
Anti myc, by Merck Group (1 mentions)
Anti ha antibody, by Santa Cruz Biotechnology (1 mentions)
Anti flag, by Merck Group (1 mentions)
Mg132, by Selleck Chemicals (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Pam3csk4, by InvivoGen (1 mentions)
Erlotinib, by MedChemExpress (1 mentions)
2 protocols using recombinant human il 36γ
1
HaCaT Cell IL-36γ Stimulation
2
Elucidating Inflammatory Signaling Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Erlotinib was purchased from MedChem Express. Cetuximab was from MERCK Serono. Trametinib and vemurafenib were from ApexBio. Selumetinib and MG132 were from Selleckchem. Recombinant human IL-36γ (catalog 6835), mouse IL-36γ (catalog 6996), and human IL-36Ra (catalog 1275) were from R&D Systems. Pam3CSK4 was from InvivoGen. The goat anti–IL-36γ (catalog AF2320) and anti-mouse KLF4 (catalog AF3158) antibodies were from R&D Systems. The rabbit anti–IL-36γ (catalog LS‑C201142) and its blocking peptide (catalog LS-E45854) were from LifeSpan BioSciences. The anti–human KLF4 antibody (catalog AM09057PU-N) was from Acris. The anti–β-actin (catalog A5441), anti-FLAG (catalog F1804), and anti-myc (catalog C3956) antibodies were from Sigma-Aldrich. The anti–human KLF4 (catalog 12173), anti-ERK (catalog 9107), anti–phospho-ERK (catalog 4370), and anti–phospho-threonine/proline (catalog 9391) antibodies were from Cell Signaling Technology. The anti-T7 antibody was from Abcam (catalog ab9138). The anti-HA antibody was from Santa Cruz Biotechnology (catalog sc-805). The secondary antibodies used were alkaline phosphatase–conjugated mouse IgG (catalog S372B), rabbit IgG (catalog S373B), and goat IgG (V115A) from Promega. Live C. acnes was prepared as previously described (63 (link)).
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