Cd45 fitc hi30

Manufactured by BD

CD45-FITC (HI30) is a fluorescently labeled antibody used for the identification and analysis of leukocytes in flow cytometry applications. It specifically binds to the CD45 antigen, which is expressed on the surface of most human white blood cells.

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Lab products found in correlation

Lsr fortessa cytometer, by BD (1 mentions) Cd3 apc cy7 sk7, by BD (1 mentions) Hladr bv510 g46 6, by BD (1 mentions) Cd8 percp cy5.5 rpa t8, by BD (1 mentions) Facs lysing solution, by BD (1 mentions) Streptavidin apc cy7, by BD (1 mentions) Cd11b apc m1 70, by Thermo Fisher Scientific (1 mentions) F4 80 pe bm8, by Thermo Fisher Scientific (1 mentions) Immersol medium, by Zeiss (1 mentions) C kit apc 2b8, by BD (1 mentions)

Close spelling variants of this product

CD45-FITC (clone HI30; CD45 (HI30, CD45-FITC

2 protocols using cd45 fitc hi30

Comprehensive Immune Cell Profiling

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Whole fresh blood samples were prepared immediately after collection for immunophenotyping. Immune cell populations were characterized by surface staining with the following fluorescence-labeled antibodies according to the manufacturers´ instructions: HLADR-BV510 (G46-6; BD Bioscience), CD3 APC Cy7 (SK7, BD Bioscience), CD45-FITC (HI30, BD Bioscience), CD14-PECF 594 (MφP9, BD Bioscience), CD19-PE Cy5 (HIB 19, BD Bioscience), CD8-PerCP CY5.5 (RPA-T8, BD Bioscience), CD4-PE Cy7 (RPA-T4, BD Bioscience), and CD56-BV785 (5.1H11, Biolegend). For the lysis of red blood cells, FACS lysing solution (BD Bioscience) was added. Viability dye (VD, eBioscience) staining was used to evaluate apoptotic cells. Cell frequencies were evaluated on the LSR Fortessa cytometer (BD Bioscience, San Jose, CA, USA). Gating strategy for cells is presented in Supplementary Figure S1.

Proschmann U., Shalchi-Amirkhiz P., Andres P., Haase R., Inojosa H., Ziemssen T, & Akgün K. (2023). Influence of exercise on quantity and deformability of immune cells in multiple sclerosis. Frontiers in Neurology, 14, 1148106.

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Comprehensive Hematopoietic Lineage Staining

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Monoclonal antibodies for lineage staining used were Gr1-PE (clone-RB6-8CS), B220-PE (RA3-6B2), Sca-1-PE (D7), ckit-APC (2B8) and CD45-FITC (HI30) from BD Biosciences, CD11b-APC (M 1/70) and F4/80-PE (BM8) from eBioscience. Antibodies used for the lineage cocktail were PerCP Cy5.5 labelled CD4 (GK1.5) and Ter119 (Ter-119) from Biolegend, B220 (RA3-6B2) and CD3 (145-2C11) from BD Biosciences and Gr-1 (RB6-8C5C), CD8 (53-6.7), IL7R (A7R34), CD11b (M1/70) from eBioscience. Antibodies used for SLAM HSC staining were CD48-APC (HM48-1), CD150-PECy7 (TC-15-12F12.2) from Biolegend and CD244-PE (2B4) from eBioscience. Antibodies used for progenitor staining were CD16/32-PE (93), CD34-biotin (RAM34), Sca1-PECy7 (D7) from eBioscience, ckit-APC (2B8) and Streptavidin-APC-Cy7 from BD Biosciences. Cytospin preparations were stained with Wright-Giemsa stain. The morphology of bone marrow was assessed with an Olympus BX51 (Olympus, Tokyo, Japan) microscope and a 40x/0.75 numerical aperture objective, or a 100x/1.3 numerical aperture objective with Zeiss immersol medium (Zeiss, Jena, Germany). OlympusXC50 (Olympus) and analySIS software (Soft Imaging System, Stuttgart, Germany) were used to capture images.

Chaturvedi A., Araujo Cruz M.M., Jyotsana N., Sharma A., Goparaju R., Schwarzer A., Görlich K., Schottmann R., Struys E.A., Jansen E.E., Rohde C., Müller-Tidow C., Geffers R., Göhring G., Ganser A., Thol F, & Heuser M. (2016). Enantiomer-specific and paracrine leukemogenicity of mutant IDH metabolite 2-hydroxyglutarate. Leukemia, 30(8), 1708-1715.

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