Trypsin 2.5

PNF was acquired from Hebei Jiahenglengbei Co., Ltd., (Yunnan; batch numbers C20071810 and C20071809, respectively). Distilled water was acquired from Guangzhou Watsons Food & Beverage Co., Ltd. D101 macroporous resin, standard saponins (ginsenosides Rb1, Rb2, Rb3, Rc, Rd, Re, Rg1, and notoginsenoside R1), and vanillin were acquired from Shanghai Yuanye Biotechnology Co., Ltd. (batch numbers B21053, B21054, B21055, B21099, B210056, B21050, B21051, and B21052, respectively). Anhydrous ethanol and glacial acetic acid were acquired from Fuchen Chemical Reagent Co., Ltd. (Tianjin, China), and chromatographic grade methanol and acetonitrile were acquired from Aladdin Reagent Co., Ltd. (Shanghai, China). Dulbecco’s modified Eagle’s medium, heat-inactivated fetal bovine serum, Dulbecco’s phosphate-buffered saline (PBS), penicillin–streptomycin, and trypsin 2.5% were acquired from Life Technologies, Inc. (Grand Island, NY, USA). β-actin, PGE-2, IL-1β, and TNF-α antibodies were acquired from Cell Signaling Technology (Beverly, MA, USA). PGE-2, IL-1β, and TNF-α enzyme-linked immunosorbent assay (ELISA) kits were purchased from BioChell (Shanghai, China). The COX-2 inhibitor screening kit was acquired from Beyotime Biotechnology (Shanghai, China).

The reagents that were used in experiments are listed below: Paraformaldehyde (P6148), Poly(ethyleneimine) solution (P3143), penicillin–streptomycin (P4333), (+)-cis-Diltiazem hydrochloride (D2521), (±)-Verapamil hydrochloride (V4629), Isradipine (I6658), Nifedipine (N7634) (Sigma-Aldrich, Saint Louis, MO, USA), Neurobasal-A medium (10888022), B-27 supplement (17504044), Trypsin 2.5% (15090046), Goat serum New Zeland origin (16210072) (Life Technologies, Grand Island, NY, USA), Fura-2 AM (F1221), Hoechst 33,342 Trihydrochloride Trihydrate (H1399) (Molecular Probes, Eugene, OR, USA), ML 218 hydrochloride (4507) (Tocris Bioscience, Bristol, UK), NBQX disodium salt (N-186), D-AP5 (D-145) (Alomone Labs, Jerusalem, Israel), Bicuculline (11727) (Cayman Chemical, Ann Arbor, MI, USA), goat anti-mouse Alexa Fluor 647 antibody (ab150115) (Abcam, Cambridge, UK), monoclonal mouse anti-GFAP antibodies (GF1 clone, L18/03) (Bialexa, Moscow, Russian Federation); Triton X-100 (Am-O694) (Amresco LLC, Solon, OH, USA); EGTA (A-0878), EDTA (A5097), (AppliChem, Darmstadt, Germany); HEPES (Cat. No 3350) (Dia-M, Moscow, Russian Federation).

The reagents that were used in the experiments are listed below. (1) Sigma-Aldrich, Saint Louis, MO, USA: Poly(ethyleneimine) solution (Cat. no. P3143), penicillin–streptomycin (Cat. no. P4333), L-Glutamine (Cat. No G85402). (2) Life Technologies, Grand Island, NY, USA: B-27 supplement (Cat. no. 17504044), Trypsin 2.5% (Cat. no. 15090046). (3) Molecular Probes, Eugene, OR, USA: Fura-2 AM (Cat. no. F1221). (4) Tocris Bioscience, Bristol, UK: UBP 310 (Cat. no. 3621), 5-Nonyloxytryptamine oxalate (Cat. No. 0901), WIN 55,212-2 mesylate (Cat. no. 1038) (5) Alomone Labs, Jerusalem, Israel: D-AP5 (Cat. no. D-145), NBQX (Cat. no. N-185). (6) Cayman Chemical, Ann Arbor, MI, USA: Bicuculline (Cat. no. 11727), HEMADO (Cat. No. 21015); (7) AppliChem, Darmstadt, Germany: EDTA (Cat. no. A5097), EGTA (Cat. no. A-0878). (8) Dia-M, Moscow, Russian Federation: HEPES (Cat. no. 3350). (9) Abcam, Cambridge, UK: N6-Cyclohexyladenosine (Cat. no. ab120472). (10) Paneco, Moscow, Russian Federation: Neurobasal-A medium.