Pluronic l64

All chemicals including Pluronic triblock copolymers (CAS 9003-11-6) with the number average molecular weights (M_ns) of ∼1100 (product name Pluronic L-31), ∼2900 (Pluronic L-64), ∼5800 (Pluronic P-123), and ∼14 600 (Pluronic F-108) were purchased from Sigma-Aldrich. Aqueous CTAB solution (0.2 M) was recrystallized twice. X-ray diffraction (XRD) patterns were obtained with Rigaku Ultima IV diffractometer using CuKα radiation (λ = 1.54056 Å) in θ–θ mode. JAC ultrasonic 1505 (150 W, 40 kHz) was used for sonication. Transmission electron microscopy (TEM) images were obtained with JEOL JEM 2010FX operating at 200 kV. Scanning electron microscopy (SEM) images were taken with JEOL JSM 6700F with an operating voltage of 5.0 kV. Fourier transform infrared (FTIR) spectra were recorded with JASCO FTIR-4600 type A, ranging from 650 cm⁻¹ to 4000 cm⁻¹ with a resolution of 4 cm⁻¹ and with an average scan of 8. Without adding KBr or making KBr pellets, sample was placed on the single-reflection ATR accessory and the spectra were measured while the pressure applicator was implemented.

Pluronic L64 and branched polyethylenimine (bPEI, Mw = 25 kDa) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tea polyphenol powder (EGCG wt% ≥80%) was obtained from Wuxi Taiyo Green Power Co. Ltd. (Jiangsu, China). DNase I and DNase I reaction buffer were obtained from Invitrogen (Carlsbad, CA, USA). HBG buffer (HEPES 20 mM, pH 7.4, 5% (w/v) glucose) and other buffers were prepared in MilliQ ultrapure water and filtered (0.22 μm) prior to use. All other chemicals were purchased from Sigma-Aldrich and used without further purification. All chemicals and reagents were of analytical grade.
The plasmids encoding β-d-galactosidase (pCMV-LacZ), luciferase (pCMV-Luc), far-red fluorescent protein (pCMV-E2) and growth-hormone-releasing hormone (pCMV-GHRH) were previously constructed in our laboratory [18 (link), 23 , 25 (link)]. The plasmids were amplified in E. coli DH5α and extracted using the Endo-Free Plasmid Kit (Invitrogen, Carlsbad, CA, USA).

Methyltrimethoxysilane (MTMS) was purchased from Shin-Etsu Chemical Co., Ltd. (Japan). Acetic acid (HOAc, ≥99.7%), distilled water (H₂O), methanol (MeOH, ≥99.5%), 2-propanol (IPA, ≥99.0%), and N,N-dimethylformamide (DMF, ≥99.5%) were purchased from Kishida Chemical Co., Ltd. (Japan). Aqueous tetramethylammonium hydroxide (TMAOH, ca. 25%) and cationic surfactant n-hexadecyltrimethylammonium chloride (CTAC) were purchased from Tokyo Chemical Ind. Co., Ltd. (Japan). Urea was purchased from Hayashi Pure Chemical Ind., Ltd. (Japan). Nonionic surfactant Pluronic F127 (EO₁₀₆PO₇₀EO₁₀₆, M_w ~ 12,600) and Pluronic L64 (EO₁₃PO₃₀EO₁₃, M_n ~ 2900) were purchased from Sigma-Aldrich Co., LLC (USA). Nonionic surfactant Synperonic P105 (EO₃₇PO₅₆EO₃₇, M_w ~ 6500) was kindly donated by Croda Japan K.K. Nonionic surfactant Pluronic P94 (EO₂₆PO₄₈EO₂₆, M_w ~ 5000) was purchased from BASF (Germany). Nonionic surfactant Pluronic F68 (EO₇₆PO₂₉EO₇₆, M_w ~ 8400) was kindly donated by ADEKA Co., Ltd. (Japan). All reagents were used as received.