Rabbit anti f4 80

Manufactured by Thermo Fisher Scientific

Sourced in United States

Rabbit anti-F4/80 is a laboratory reagent that recognizes the murine F4/80 antigen, a membrane glycoprotein expressed on the surface of macrophages. This reagent can be used in various immunological techniques, such as flow cytometry and immunohistochemistry, to identify and characterize macrophage populations.

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Lab products found in correlation

Mouse anti f4 80, by Santa Cruz Biotechnology (1 mentions) Anti mouse cd206, by Abcam (1 mentions) Lsm 780 confocal microscope, by Zeiss (1 mentions) Mouse anti cd68, by Novus Biologicals (1 mentions) Goat anti mouse igg alexa fluor 488 conjugate, by Thermo Fisher Scientific (1 mentions) Image it fx signal enhancer, by Thermo Fisher Scientific (1 mentions) Rabbit anti nrf2, by Santa Cruz Biotechnology (1 mentions) Confocal laser scanning microscopy, by Leica (1 mentions) Goat anti rabbit igg alexa fluor 647 conjugate, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

F4/80 (353 citations) Anti-F4/80 (122 citations)

2 protocols using rabbit anti f4 80

Immunofluorescent Lung Tissue Analysis

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Formalin-fixed, paraffin-embedded lung tissue sections (left lung lobe, 5 μm) were deparaffinized, antigen-unmasked by heating tissue sections in universal antigen retrieval reagent (Cell Signaling Technology, Danvers, MA), and used to perform immunofluorescent staining. The primary antibodies used were rabbit anti-F4/80 (1:100, Thermo Fisher Scientific) or mouse anti-F4/80 (1:100, Santa Cruz Biotechnology, Santa Cruz, CA), mouse anti-CD68 (1:100, Novus Biologicals, Centennials, CA), mouse anti-CD206 (1:100, Abcam, Cambridge, MA, USA), rabbit anti-ALOX5AP (1:100, Abcam), and mouse anti-ALOX15 (1:200, Abcam) antibodies. Images were taken in three to five randomly selected fields per lung slice, three lung slices per mouse, using a Zeiss LSM 780 confocal microscope with a 63× magnification lens (Carl Zeiss Microscopy, Jena, Germany). More than 500 cells from captured microscopic images per each treatment were counted using the ImageJ software (NIH, Bethesda, USA) and the number of cells with double positive staining per every 100 cells were presented as mean ± SEM (n = 3).

Lim C.S., Porter D.W., Orandle M.S., Green B.J., Barnes M.A., Croston T.L., Wolfarth M.G., Battelli L.A., Andrew M.E., Beezhold D.H., Siegel P.D, & Ma Q. (2020). Resolution of Pulmonary Inflammation Induced by Carbon Nanotubes and Fullerenes in Mice: Role of Macrophage Polarization. Frontiers in Immunology, 11, 1186.

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Immunofluorescence Staining of YAMC and Tissue

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For YAMC staining, cells were fixed with 4% paraformaldehyde for 10 min and permeabilized with pre-cold methanol for 10 min at −20 °C. Slides were blocked using Image-iT^® FX Signal Enhancer (Life Technologies) for 30 min and then 5% normal goat serum for 1 h at room temperature. Mouse anti-GSTA4 (Abnova; H00002941-B01P, 1:100) and rabbit anti-Nrf2 (Santa Cruz Biotechnology; sc-722, 1:400) polyclonal antibodies, rabbit anti-F4/80 (Thermo Scientific, Waltham, MA, USA; MA5-16363, 1:100) and mouse anti-phospho-c-Jun (Ser⁷³) (Santa Cruz Biotechnology; sc-822, 1:400) monoclonal antibodies were incubated at 4 °C overnight. Goat anti-mouse IgG-Alexa Fluor^® 488 conjugate and goat anti-rabbit IgG-Alexa Fluor^® 647 conjugate (Life technologies; A11029 and A21244, 1:1,000) were used as secondary antibodies. DNA was counterstained with 4’,6-diamidino-2-phenylindole (DAPI). To stain F4/80 and GSTA4 in formalin-fixed, paraffin-embedded tissues, epitope retrieval was conducted as described in the IHC staining followed by blocking and staining protocols as for YAMC staining. Images were analyzed by laser scanning confocal microscopy (Leica Microsystems, Buffalo Grove, IL, USA).

Yang Y., Huycke M.M., Herman T.S, & Wang X. (2016). Glutathione S-transferase Alpha 4 Induction by Activator Protein 1 in Colorectal Cancer. Oncogene, 35(44), 5795-5806.

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