Anti cd44

Manufactured by Bioss Antibodies

Anti-CD44 is a monoclonal antibody that targets the CD44 antigen. CD44 is a cell surface glycoprotein involved in cell-cell interactions, cell adhesion, and migration. The Anti-CD44 antibody can be used for the detection and analysis of CD44 expression in various biological samples and research applications.

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Lab products found in correlation

Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti mouse ck 14, by BioLegend (1 mentions) Anti p21, by Bioss Antibodies (1 mentions) Anti s nitrosocysteine, by Abcam (1 mentions) Anti human ck 14, by Thermo Fisher Scientific (1 mentions) Anti human ck 18, by Thermo Fisher Scientific (1 mentions) Anti dynll1, by Abcam (1 mentions) Anti erbb2, by Thermo Fisher Scientific (1 mentions) Anti gm130, by Cell Signaling Technology (1 mentions) Anti p27, by Abcam (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti cd45, by Bioss Antibodies (1 mentions) Anti cd34, by Bioss Antibodies (1 mentions) Anti cd31, by Bioss Antibodies (1 mentions) Anti cd90, by Bioss Antibodies (1 mentions) Anti vimentin, by Bioss Antibodies (1 mentions) Kaluza 2, by Beckman Coulter (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Cytoflex, by Beckman Coulter (1 mentions)

3 protocols using anti cd44

Comprehensive Antibody Protocol for Cell Analysis

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The following antibodies were used. Anti-β-Galactosidase (Bioss, bs-4960R), anti-alkaline phosphatase (Novus Biologicals, NBP1-32948); anti-heterochromatin protein 1-γ (HP1-γ, phospho Ser 93, Bioss, bs-3221R); anti-p21 (Bioss, bs-10129R); anti-p27 (phospho Thr 187, Abcam, ab75908); anti-NOS-1, 2 (Thermo Fisher Scientific, PA1-033 and -036); anti-NOS-3 (Sigma-Aldrich, SAB-4300435); anti-ErbB2 (Thermo Fisher Scientific, PA5-16395); anti-pSMAD3 (Ser423/Ser425, Novous Biological, NBP1-77836); anti-CD24 (Novus Biologicals, NBP1-4639055); anti-CD44 (Bioss, bs-2507R); anti-S-Nitroso-Cysteine (Abcam, ab50185 or Alpha Diagnostics, NISC11-A); anti-Integrin α6 (BD Biosciences, 555734); anti-GM130 (Cell Signaling, 12480 S); anti-human CK 14 (ThermoFisher, MA511599); anti-human CK 18 (ThermoFisher, PA514263); anti-mouse CK 14 (BioLegend, 905301); anti-human CK 8/18 (DSHB, Troma-I); anti-Cleaved Caspase3 (Cell Signaling, #9664); anti-β-Actin (Sigma, A1978); anti-DYNLL1 (Abcam, ab51603); and anti-ADMA (EMD Millipore, 09-814).

Ren G., Zheng X., Bommarito M., Metzger S., Walia Y., Letson J., Schroering A., Kalinoski A., Weaver D., Figy C., Yeung K, & Furuta S. (2019). Reduced Basal Nitric Oxide Production Induces Precancerous Mammary Lesions via ERBB2 and TGFβ. Scientific Reports, 9, 6688.

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Flow Cytometry Analysis of Stem Cell Markers

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BMSC and ADSC cell surface markers were examined via flow cytometry using the forward scatter/side scatter method. Cells were trypsinized and resuspended in PBS at 1x10⁵ cells per 1.5 ml. BMSCs were incubated for 2 h at room temperature in the dark with the following FITC-conjugated antibodies: Anti-CD29 (1:50; cat. no. bs-20630R-FITC), anti-CD90 (1:200; cat. no. bs-0778R-FITC), anti-CD45 (1:100; cat. no. bs-10599R-FITC) and anti-CD34 (1:50; cat. no. bs-0646R-FITC; all from BIOSS). ADSCs were incubated for 2 h at room temperature with the following FITC-conjugated antibodies: Anti-rat stem cells antigen-1 (Sca-1; 1:100; cat. no. bs-3752R-FITC; BIOSS), anti-CD44 (1:50; cat. no. bs-0521R-FITC), anti-CD45 (1:100; cat. no. bs-10599R-FITC) and anti-CD11b (1:200; cat. no. bs-1014R-FITC; all from BIOSS). Subsequently, the cells were washed twice with PBS and analyzed by a CyFlow™ Space flow cytometer (Sysmex Partec GmbH) with FloMax 2.8 software (Sysmex Partec GmbH).

Dai X., Guan Y., Zhang Z., Xiong Y., Liu C., Li H, & Liu B. (2021). Comparison of the differentiation abilities of bone marrow-derived mesenchymal stem cells and adipose-derived mesenchymal stem cells toward nucleus pulposus-like cells in three-dimensional culture. Experimental and Therapeutic Medicine, 22(3), 1018.

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Immunophenotyping of Rat Mesenchymal Stem Cells

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The immunophenotype of rat MSCs was evaluated by flow cytometry analysis performed on CytoFlex (Beckman Coulter). Cells were resuspended in 100 μL of PBS containing 1% of bovine serum albumin (Sigma-Aldrich, Saint Luis, MO), incubated for 20 minutes at 20°C in the darkness with the following monoclonal antibodies: anti-CD90, anti-CD44, anti-CD31, anti-CD34, anti-CD45 and anti-vimentin (Bioss). Unstained cells of corresponding control isotype antibodies were used as a negative control. A threshold was set to a forwardscatter (FSC) parameter to exclude cell debris. The SSC (side-scatter) and FSC settings were done with logarithmic amplification scale as well as fluorescence channels and dot plot analysis. 10 000 of target events were analysed. For data analysis, Kaluza 2.0 software (Beckman Coulter) was used.

Karpov A.A., Puzanov M.V., Ivkin D.Y., Krasnova M.V., Anikin N.A., Docshin P.M., Moiseeva O.M, & Galagudza M.M. (2019). Non-inferiority of microencapsulated mesenchymal stem cells to free cells in cardiac repair after myocardial infarction: A rationale for using paracrine factor(s) instead of cells. International journal of experimental pathology, 100(2).

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