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3 protocols using cysteamine hydrochloride

1

Stimuli-responsive Polymer Nanocarriers

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The mPEG4000 was purchased from Best-reagent (Beijing, China). N-Carbobenzoxy-l-glutamic acid (PBLG) was purchased from Wuxi Asiapeptide Biotechnology Co., Ltd. (Wuxi, China). Acryloyl chloride, cysteamine hydrochloride, and DOX·HCl were purchased from Macklin (Shanghai, China). 4,6-Diamidino-2-phenylindole (DAPI), 1,10-dioctadecyl-3,3,30,30 tetramethylindodicarbocyanine perchlorate (DiD), and LysoTracker Green were purchased from Invitrogen (Carlsbad, CA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Kumamoto, Japan). Hoechst 33342 was purchased from BD Biosciences (San Jose, CA).
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2

Synthesis and Functionalization of Multifunctional Nanoparticles

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Cetyltrimethylammonium bromide (CTAB, 99%), disodium hydrogen phosphate (Na2HPO4, AR), and sodium dihydrogen phosphate (NaH2PO4, AR) were purchased from Shanghai Titan Company. Tetraethyl orthosilicate (TEOS, 99%) was purchased from Saen Chemical Technology (Shanghai) Company. (3-Aminopropyl) triethoxysilane (KH550, 98%) was purchased from Shanghai Xushuo Biotechnology Co., Ltd. N-hydroxysulfonylsuccinimide (NHS, 98%), 1-ethyl-(3-methylaminopropyl) carbodiimide hydrochloride (EDC·HCl 99%), glutathione (reduced type, GSH, 98%), and cysteamine hydrochloride (Cys·HCl, 98%) were purchased from Shanghai Macklin Biochemical Company. Tris(2-chloroethyl) phosphate (TCEP) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. Doxorubicin hydrochloride (DOX·HCI, 98%) was purchased from J&K. Sodium cyanoborohydride (NaBH3CN, 95%) was purchased from Shanghai Jiuding Chemical Technology Company. Sodium hyaluronate (low-molecular-weight NaHA, MW: 3000, 11 000) was purchased from Shanghai Yuanye Biological Company. 42nt-oligomeric acid (HApt-SH, 5′-SH-GCAGCGGTGTGGGG/GCAGCGGTGTGGGG/GCAGCGGTGTGGGG-3′, AR) and Texas Red-42nt-oligomeric acid (Texas Red-HApt-SH, AR) were purchased from Sangon Biotech (Shanghai) Company. Ultrapure water (H2O, 18.2 MΩ) and high purity nitrogen (N2, high purity) were used throughout the study.
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3

Electrochemical Biosensor for Influenza A H1N1

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The HAuCl4·3H2O, KNO3, cysteamine hydrochloride (Cys), glutaric dialdehyde (Glu), bovine serum albumin (BSA), and sodium citrate were purchased from Shanghai Macklin Biochemical Co., Ltd. Recombinant Hemagglutinin-Influenza A Virus H1N1 California 04/2009 was purchased from Prospec-Tany TechnoGene Co., Ltd (Ness-Ziona, Israel). H1N1 Hemagglutinin 1 was purchased from Beijing Bioss Co., Ltd. Ethyl alcohol was purchased from Sigma-Aldrich, USA. The Ag/AgCl Carbon Paper (CP) and Pt electrodes were purchased from Tianjin Lanlike Chemical Electronic High Technology Co., LTD. Phosphate-buffered saline (PBS) was purchased from Sigma-Aldrich, USA.
Electrochemical measurements were conducted at an ambient temperature using a CHI 660E electrochemical workstation (CH Instruments, Inc., USA). Deionized water (~18.0 MΩ) was obtained from a Millipore system.
All synthetic materials and electrodes were characterized using the following tools: scanning electron microscopy (SEM) was performed using a Nova Nano SEM 430 scanning electron microscope.
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