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Dtslp0

Manufactured by R&D Systems
Sourced in United States

The DTSLP0 is a laboratory instrument designed to measure the dissociation time and solubility of test compounds. It provides quantitative data on the rate of dissolution and the degree of solubility under controlled conditions. The core function of this product is to assist researchers in evaluating the physical and chemical properties of materials relevant to pharmaceutical and other industries.

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2 protocols using dtslp0

1

Quantitative ELISA for TSLP, TGFβ, and IL-10

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Samples, including peripheral blood serum, tissue homogenates or cultured cell supernatants, were collected for quantitative ELISAs of TSLP (R&D Systems, DTSLP0 for human, MTLP00 for mouse), TGFβ (R&D Systems, SB100B) and IL-10 levels (eBioscience, 88-7106 for human, 88-7105 for mouse) according to the manufacturer's instructions.
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2

Cytokine Profiling in Cell-Free Sputum Supernatants

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In cell-free sputum supernatants, the following cytokines were measured with ELISA kits according to the manufacturer's instructions: IL-1β (ab46052; Abcam, Cambridge, UK), IL-5 (ab46026; Abcam), IL-6 (KHC0061; Invitrogen, Camarillo, CA, USA), IL-8 (ab46032; Abcam), IL-13 (ab46038; Abcam), IL-17 (KAC1591; Invitrogen), IL-25 (EK0793; Boster Biotech; Pleasanton, CA, USA), IL-33 (SEB980Hu; Cloud-Clone Corp., Katy, TX, USA), TNF-α (ab46087; Abcam), TSLP (DTSLP0; R&D Systems, Minneapolis, MN, USA), GM-CSF (HCYTOMAG-60K; EMD Millipore, Darmstadt, Germany), and IFN-γ (HCYTOMAG-60K; EMD Millipore). Cytokine levels were normalized to protein concentrations in the sputum.
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