Rna reagents

Cell Counting Kit-8 (CCK-8) was provided by Dojindo Co. (Kumamoto, Japan). Puromycin and polybrene were provided by Sigma-Aldrich Chemicals (St. Louis, Mo). Antibodies for HBO1 (#58418), cleaved caspase-3 (#9664), acetyl-Histone H3 at Lys14 (H3K14ac, #7627), Histone H3 (#4499), acetyl-Histone H4 at Lys12 (H4K12ac, #13944), Histone H4 (#2935), acetyl-Histone H4 at Lys5 (H4K5ac, #8674), cleaved-poly (ADP-ribose) polymerase (PARP) (#5625), cleaved-caspase-9 (#20750), Caspase-3 (#9668), Caspase-9 (#9508), PARP (#9532) and Tubulin (#2125) were obtained from Cell Signaling Tech China (Shanghai, China). A Zinc finger protein 384 (ZNF 384) antibody was provided by Abcam (ab176689, Shanghai, China). Caspase inhibitors, z-DEVD-fmk and z-VAD-fmk, were provided by Sigma-Aldrich Chemicals. The HBO1 inhibitor WM-3835, N'-(4-fluoro-5-methyl-[1,1'-biphenyl]-3-carbonyl)-3-hydroxybenzenesulfonohydrazide was synthesized by Min-de Biotech (Suzhou, China) based on the described protocol 33 (link). RNA reagents, Lipofectamine 2000, and other transfection reagents were provided by Thermo-Fisher Invitrogen (Shanghai, China). All primers, sequences, constructs, and vectors were designed and provided by Genechem Co. (Shanghai, China), unless mentioned otherwise.

Puromycin and polybrene were provided by Sigma-Aldrich Chemicals (St. Louis, Mo). All the antibodies were obtained from Abcam (Cambridge, MA). RNA reagents and Lipofectamine 2000 were obtained from Thermo-Fisher Invitrogen (Shanghai, China). All sequences and plasmids were provided by Shanghai Genechem Co. (Shanghai, China), unless otherwise mentioned.