The blood samples were obtained between 8.30 and 9.30 a.m. after fasting for 8 h and having slept for 6 h. The samples were processed in a clinical analysis laboratory to measure serum cortisol levels by Elecsys Cortisol II electrochemiluminescence immunoassay “ECLIA” using the Cobas 6000 instrument (Roche Diagnostics GmbH, Germany).
Cortisol 2
Manufactured by Roche
Sourced in Germany
Cortisol II is a laboratory equipment product. It is used for the quantitative determination of cortisol in human serum and plasma.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using cortisol 2
1
Serum Cortisol Measurement Protocol
2
Serum Cortisol and ACTH Levels in Patients
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Serum cortisol levels and ACTH levels were analyzed in all patients. LCR was defined by a random cortisol level < 14.6 μg/dL (=403 nmoL/L).23 , 24 , 25 Baseline concentration reference level for ACTH were 10–50 pg/mL (2.2–11 pmoL/L). Cortisol level and ACTH level were measured by electrochemiluminescence with the Cortisol II and Elecsys® ACTH immunoassay (Roche Diagnostics).
3
Quantification of Endocannabinoids and Cortisol in Plasma
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Blood samples were taken both pre- and posttreatment between 08.00 and 11.00 AM
All blood samples were collected between 2010–2013. After collection of the
blood samples, they were immediately centrifuged to extract plasma and frozen
at − 80 °C. In 2019 AEA and 2-AG levels were determined. Anandamide levels were
determined using the AEA ELISA (abx258779, Abbexa Ltd, Cambridge, UK). The lower
limit of detection was 3 ng/mL. Intra-assay variation at 60 ng/mL was 3.2%.
Inter-assay variation at 60 ng/mL was 8.8%. Arachidonoylglycerol levels were
determined using the 2-AG ELISA (abx258337, Abbexa Ltd, Cambridge, UK). The
lower limit of detection was 4 ng/mL. Intra-assay variation at 95 ng/mL was
8.4%. Inter-assay variation at 95 ng/mL was 11.0%. Additionally, we determined
cortisol levels because of the role of the ECS in mediating the actions of glucocorticoids.10 (link)
Cortisol was measured in one batch using an electrochemiluminescence
immunoassay on the Modular E411 (Cortisol II, Roche Diagnostics GmbH,
D-68298 Mannheim, Germany). The lower limit of detection was 2 nmol/L and intra
assay variation was <2.4% at 100–900 nmol/L respectively.
All blood samples were collected between 2010–2013. After collection of the
blood samples, they were immediately centrifuged to extract plasma and frozen
at − 80 °C. In 2019 AEA and 2-AG levels were determined. Anandamide levels were
determined using the AEA ELISA (abx258779, Abbexa Ltd, Cambridge, UK). The lower
limit of detection was 3 ng/mL. Intra-assay variation at 60 ng/mL was 3.2%.
Inter-assay variation at 60 ng/mL was 8.8%. Arachidonoylglycerol levels were
determined using the 2-AG ELISA (abx258337, Abbexa Ltd, Cambridge, UK). The
lower limit of detection was 4 ng/mL. Intra-assay variation at 95 ng/mL was
8.4%. Inter-assay variation at 95 ng/mL was 11.0%. Additionally, we determined
cortisol levels because of the role of the ECS in mediating the actions of glucocorticoids.10 (link)
Cortisol was measured in one batch using an electrochemiluminescence
immunoassay on the Modular E411 (Cortisol II, Roche Diagnostics GmbH,
D-68298 Mannheim, Germany). The lower limit of detection was 2 nmol/L and intra
assay variation was <2.4% at 100–900 nmol/L respectively.
4
Salivary Cortisol Measurement Protocol
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The methods and parameters to collect and analyze salivary cortisol level referred to previous human stress literature (Sokol-Hessner et al., 2016; (link)van den Bos et al., 2009; (link)Yao et al., 2016) (link). We used the salivate collection tubes (Sarstedt, Rommelsdorf, Germany) to collect saliva samples. Participants need to hold a small piece of sterile tampon in their mouth for about 1-2 min until it was completely soaked. We reserved the saliva samples at À22 C until assayed by ECLIA (Cortisol II, Roche Diagnostics, Numbrecht, Germany). The lower sensitivity for cortisol was 1.5 nmol/L, and the detectable range was 1.5-1750 nmol/L. Intra-and inter-assay variations were below 7.1% and 12.7%, respectively.
5
Comprehensive Metabolic Panel Analysis
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Urea and glucose concentrations were measured by kinetic colorimetric assays using the UREAL (Roche Diagnostics, Cat.# 05171873 190) and GLUC3 kit (Roche Diagnostics, Cat.# 05168791 190). Cholesterol, TRIGLycerides and lipoprotein concentrations were measured via enzymatic color assays using the CHOL2 (Roche Diagnostics, Cat.# 05168538 190), TRIGL (Roche Diagnostics, Cat.# 05171407 190), HDLC4 kit (Roche Diagnostics, Cat.# 07528582 190) and LDLC3 kit (Roche Diagnostics, Cat.# 07005768 190). These analyses were performed on a Cobas 8000/c702 system (Roche Diagnostics) according to the instructions of the manufacturer. Cortisol, Insulin and c-peptide concentrations were measured via electrochemiluminescence immunoassays (ECLIA) using the Cortisol II (Roche Diagnostics, Cat.# 07027150 190), Insulin (Roche Diagnostics, Cat.# 07027559 190) and C-Peptide kit (Roche Diagnostics, Cat.# 07027168 190) on a Cobas 8000/e801 system (Roche Diagnostics) according to the instructions of the manufacturer.
6
Progesterone and Cortisol Quantification in Adrenocortical Cells
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For progesterone determination, transfected and silenced Y1 adrenocortical tumor cells were incubated for 24 h with forskolin 10 mM. To test the effects of Y27632, cells were treated with 10 mM forskolin with or without 2.5 mM Y27632 for For cortisol determination, human primary adrenocortical tumor cells were treated with 10 mM forskolin with or without 2.5 mM Y27632 for 18 h and then analysed by a specific immunoassay (Cortisol II, Cobas, Roche, Basel, Switzerland).
A pre-incubation with Y27632 for 30 min was performed prior to Y27632 incubation.
A pre-incubation with Y27632 for 30 min was performed prior to Y27632 incubation.
7
Saliva Cortisol Response Assessment
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Saliva was used to test for cortisol responses due to its ease of compliance, low invasiveness, and ability to track the biologically active "free" hormone . Saliva (2ml) was collected by passive drool into sterile containers, and these were stored at -80 °C until assay. A saliva sample was taken before the 45-min task, after the 45-min task and after the endurance task. The saliva samples were analysed in duplicate using the "Cortisol II" test of Roche on the Cobas e601 analyser.
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