Ab126832

Manufactured by Abcam

Sourced in United Kingdom

Ab126832 is a primary antibody product manufactured by Abcam. This antibody is designed to detect a specific target protein. The product details and intended use are provided in the official product documentation and should be consulted for more information.

Automatically generated - may contain errors

Lab products found in correlation

Ab32364, by Abcam (2 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Ab20034, by Abcam (1 mentions) Mouse anti beta actin, by Proteintech (1 mentions) Enhanced chemi luminescence (ecl), by Bio-Rad (1 mentions) Ab188470, by Abcam (1 mentions) Ab2851, by Abcam (1 mentions) Ab109245, by Abcam (1 mentions) Ab6802, by Abcam (1 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Dnmt3a, by Abcam (1 mentions) Dnmt3b, by Abcam (1 mentions)

2 protocols using ab126832

Quantification of CD27+/- Treg Cells

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The quantity of CD27^+/− Tregs was measured using a BCA Protein Assay
Kit (23227; Thermo Fisher Scientific, Lithuania). The antibodies used in this
study were mouse anti-human Foxp3 (1:1,000, #ab20034; Abcam, UK), rabbit
anti-Helios (1:1,000, #42427T; CST, USA), rabbit anti-STAT5 (1:1,000, #ab126832;
Abcam, UK), rabbit anti-phosphorylated-STAT5 (p-STAT5, 1:1,000, #ab32364; Abcam,
UK), rabbit anti-JAK3 (1:1000, #8827T; CST, USA), and rabbit
anti-phosphorylated-JAK3 (1:1000, #8827S; CST, USA). Mouse anti-beta-actin
(1:5,000, #66009-1-Ig; Proteintech, Rosemount, IL, USA) was used as an internal
control. The blots were visualized using enhanced chemiluminescence (ECL;
BioRad, California, USA). Band intensity was analyzed using Quantity One
software.

Cao L., Ma X., Zhang J., Yang M., He Z., Yang C., Li S., Rong P, & Wang W. (2023). CD27-Expressing Xenoantigen-Expanded Human Regulatory T Cells Are Efficient in Suppressing Xenogeneic Immune Response. Cell Transplantation, 32, 09636897221149444.

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Western Blot Analysis of Epigenetic Regulators

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Total proteins were extracted by RIPA buffer, and BCA protein assay kit was used to determine the corresponding concentration. Protein (25 μg) was isolated by 8% SDS–PAGE and subsequently transferred to PVDF membranes. The 1% BSA in TBS buffer was used to block the membranes, and primary antibodies were cultivated at 4°C overnight. The membrane was then washed with 1× TBST and cultivated with a secondary antibody horseradish-peroxidase-conjugated in 1×TBS at room temperature for 1 h. The membrane was washed with 1× TBST, and the protein expression was determined using SuperSignal West Pico Chemiluminescent Substrate (Pierce Biotechnology). The loading control was β-actin, detected on the same blot. All primary antibodies were purchased from Abcam: DNMT3A (ab188470; 1:2,000), DNMT3B (ab2851; 1:2,000), SOCS2 (ab109245; 1:5,000), STAT5 (ab126832; 1:1,000), P-STAT5 (ab32364; 1:1,000), and the secondary antibody (ab6802, 1:2,000).

Wang K., Gong D., Qiao X, & Zheng J. (2023). MiR-532-3p inhibited the methylation of SOCS2 to suppress the progression of PC by targeting DNMT3A. Life Science Alliance, 6(5), e202201703.

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