Single-cells suspension of LPMC were depleted of CD3+ cells using CD3ɛ MicroBead Kit (130-094-973, Miltenyi Biotec) and LS columns (130-042-401, Miltenyi Biotec) and stained with anti-mouse CD19 PerCpCy5.5 (115534, Biolegend, San Diego, CA), anti-mouse CD3 APC-eFluor 780 (47-0032-82, Invitrogen), anti-mouse IgA PE (12-4204-83, Invitrogen), anti-mouse CD103 APC (17-1031-82, Invitrogen) and anti-mouse Integrin B7 BV421 (564283, BD Bioscience, La Jolla, CA) and sorted on a fluorescence-activated cell sorter FACSAria (BD Biosciences) using FACSDiva software (BD Biosciences).
Anti mouse cd3 apc efluor 780
Manufactured by Thermo Fisher Scientific
Sourced in Canada
The Anti-mouse CD3 APC-eFluor 780 is a fluorochrome-conjugated antibody that binds to the CD3 antigen expressed on mouse T cells. It is a tool used in flow cytometry applications for the identification and analysis of mouse T cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Anti mouse cd19 percpcy5, by BioLegend (2 mentions)
Anti mouse iga pe, by Thermo Fisher Scientific (2 mentions)
Anti mouse cd103 apc, by Thermo Fisher Scientific (2 mentions)
Anti mouse integrin b7 bv421, by BD (2 mentions)
Ls column, by Miltenyi Biotec (2 mentions)
Facsdiva software, by BD (2 mentions)
Facsaria, by BD (2 mentions)
Cd3ε microbead kit, by Miltenyi Biotec (1 mentions)
2 protocols using anti mouse cd3 apc efluor 780
1
Isolation and Characterization of Intestinal B Cells
2
Isolation and Sorting of Intestinal Lymphocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Single-cells suspension of LPMC were depleted of CD3+ cells using CD3ε MicroBead Kit (130-094-973, Miltenyi Biotec) and LS columns (130-042-401, Miltenyi Biotec) and stained with anti-mouse CD19 PerCpCy5.5 (115534, Biolegend, San Diego, CA), anti-mouse CD3 APC-eFluor 780 (47-0032-82, Invitrogen), anti-mouse IgA PE (12-4204-83, Invitrogen), anti-mouse CD103 APC (17-1031-82, Invitrogen) and anti-mouse Integrin B7 BV421 (564283, BD Bioscience, La Jolla, CA) and sorted on a fluorescence‐activated cell sorter FACSAria (BD Biosciences) using FACSDiva software (BD Biosciences).
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