Mouse monoclonal anti-β-actin (ab1906) was obtained from Abcam (Paris, France). The mouse monoclonal anti-core (MA1-080) antibody was obtained from ThermoFisher Scientific (Waltham, MA, USA). Mouse monoclonal anti-RACK1 (sc-17754) was obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA 95060, USA). The secondary antibody anti-mouse IgG (NXA931) coupled with HRP was obtained from Amersham, GE Healthcare.
Anti mouse igg nxa931
Manufactured by Cytiva
Anti-mouse IgG (NXA931) is a secondary antibody reagent used in immunoassays and other immunological techniques to detect the presence of mouse immunoglobulin G (IgG) in samples. It is designed to bind specifically to mouse IgG antibodies, allowing for their visualization or quantification.
Automatically generated - may contain errors
Lab products found in correlation
Mouse monoclonal anti β actin ab1906, by Abcam (2 mentions)
Mouse monoclonal anti core ma1 080 antibody, by Thermo Fisher Scientific (2 mentions)
Mouse monoclonal anti rack1 sc 17754, by Santa Cruz Biotechnology (2 mentions)
Adiponectin, by Cell Signaling Technology (1 mentions)
P hsl s563, by Cell Signaling Technology (1 mentions)
P ampkαt172, by Cell Signaling Technology (1 mentions)
Pcreb s133, by Cell Signaling Technology (1 mentions)
Perilipin 1, by Cell Signaling Technology (1 mentions)
Pparα sc 9000, by Santa Cruz Biotechnology (1 mentions)
Ha tag, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
3 protocols using anti mouse igg nxa931
1
Western Blot Antibody Validation
2
Western Blot Antibody Validation
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Mouse monoclonal anti-β-actin (ab1906) was obtained from Abcam (Paris, France). The mouse monoclonal anti-core (MA1-080) antibody was obtained from ThermoFisher Scientific (Waltham, MA, USA). Mouse monoclonal anti-RACK1 (sc-17754) was obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA 95060, USA). The secondary antibody anti-mouse IgG (NXA931) coupled with HRP was obtained from Amersham, GE Healthcare.
3
Adiponectin Regulation of Lipolysis
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Immunoblots were conducted on 3 to 7 samples randomly chosen within each experimental cohort with antibodies against HSL (#4107 or #18381), p-HSL (S563) (#4139), p-HSL (S565) (#4137), p-HSL (S660) (#4126), Perilipin-1 (#9349), p-AMPKα (T172) (#2535), AMPKα (#2532), p-CREB (S133) (#9198), CREB (#9197), p-(Ser/Thr) PKA substrate (#9621), HA-tag (#3724), Adiponectin (#2789) and β-actin (#4970) from Cell Signaling (Denvers, MA); PHLPP1, (A300-660A), PHLPP2 (A300-661A) from Bethyl Laboratories, Inc (Montgomery, TX); PPARα (SC-9000) from Santa Cruz Biotechnology (Dallas, TX). Mouse serum was neutralized by an Adiponectin antibody (ab3455) from Abcam46 (link). All primary antibodies were used at a dilution of 1:1000. HRP-linked anti-rabbit IgG (NA934) and anti-mouse IgG (NXA931) were purchased from Amersham and used at a dilution of 1:5000. Alexa Fluor 594 anti-Rabbit IgG (A32754) was purchased from ThermoFisher and used at a dilution of 1:1000.
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