Ac α tubulin

Vincristine, propidium iodide, anti-β-tubulin, FITC-conjugated anti-mouse IgG, poly-L-lysine hydrobromide, and SP600125 were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Suberoylanilide hydroxamic acid (SAHA) and 21-900 (HDAC/tubulin inhibitor) were synthesized by Dr. Jing-Ping Liou (School of Pharmacy, College of Pharmacy, Taipei Medical University, Taiwan). The above drugs were dissolved in DMSO (dimethylsulfoxide) and then stored at −20 °C. The acetyl-Histone H3, cdk1, caspase 3, PLK1 (p-Thr210), cyclin B1, cdk1/cdc2 (p-Tyr15), and caspase 7 antibodies were all purchased from BD Biosciences (San Jose, CA, USA). Caspase 8, PARP, MPM2 (pSer/pThr), PLK1, and α-actin were purchased from Millipore (Bedford, MA, USA). Aurora A, p-JNK, BID, BCL-2, p-BCL-2, MCL-1, BAX, BAK, Ac-α-tubulin, caspase 9, and cleaved caspase 3 were purchased from Cell Signaling Technologies (Beverly, MA, USA). The labeled secondary antibodies goat anti-mouse IgG-HRP and goat anti-rabbit IgG-HRP were purchased from Santa Cruz (Santa Cruz, CA, USA).

Dulbecco’s Modified Eagle’s medium (DMEM) with L-glutamine was purchased from GenDEPOT (Barker, TX, USA) and RPMI 1640 medium, fatal bovine serum (FBS), and penicillin/streptomycin were purchased from Gibco BRL (Gaithersburg, MD, USA). Antibodies specific for α-tubulin, Ac-α-tubulin, Histone H3, Ac-Histone H3, PARP, caspase 3, cleaved caspase 8, β-actin HDAC1, and HDAC6 were purchased from Cell Signalling Technology (Boston, MA, USA). Rad52 antibody and goat anti-rabbit IgG horseradish peroxidase conjugate were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Cell Titre 96 Aqueous One Solution cell proliferation assay kit was purchased from Promega (Madison, WI, USA). Amersham ECL select Western blotting detection reagent was purchased from GE Healthcare (Waukesha, WI, USA). HDAC fluorogenic assay kits (HDAC1, HDAC3, HDAC6, and HDAC8) were purchased from BPS Bioscience (San Diego, CA, USA). OxiSelect™ Comet Assay Kit was purchased from Cell Biolabs, Inc (San Diego, CA, USA).