Ten larvae from the group tested in the buoyancy assay (including both floaters and sinkers) were collected, frozen in liquid nitrogen, and weighed as a group. Larvae were homogenized and neutral lipids were extracted and analyzed as previously described [16 (link), 85 (link)] using a Thermo Fisher Trace 1300-ISQ GC/MS system. n = 8. ANOVA was used to calculate statistical significance with Prism 6 software.
Trace 1300 isq gc ms system
Manufactured by Thermo Fisher Scientific
Sourced in China
The Trace 1300-ISQ GC-MS system is a gas chromatography-mass spectrometry (GC-MS) instrument designed for analytical applications. It combines gas chromatography separation with mass spectrometry detection to provide qualitative and quantitative analysis of complex samples. The system is capable of identifying and measuring trace-level compounds in a variety of matrices.
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3 protocols using trace 1300 isq gc ms system
1
Lipid Analysis of Buoyant Larvae
2
Electrospun Nanofiber Characterization
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Thermo Trace 1300-ISQ GC-MS system was used to analyze the SCFAs, while a high-voltage power supply (DW-P403-1AC, Tianjin, China) and a syringe pump were used for the electrospinning. A field emission scanning electron microscope (FESEM, Zeiss Ultra Plus) was utilized to investigate the morphology and structure of the prepared nanofiber mats. Fourier transform infrared (FTIR) spectra were recorded using a Thermo Nicolet iS5 spectrometer (Madison, WI, USA) in the range of 400–4000 cm−1.
3
GC-MS Analysis of Fatty Acid Methyl Esters
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After methylation, the FAME were analyzed using GC-MS (Liu et al., 2021) (link) using the Trace 1300-ISQ GC-MS system (Thermo Fisher) equipped with a DB-WAX column (30 m × 0.25 mm × 0.25 µm, Agilent Technologies). The initial column temperature was 100°C and maintained for 2 min. The temperature then increased to 300°C at a rate of 10°C/min and was maintained at this temperature for 5 min. The injector was operated at 300°C, and the detector was operated at 230°C. The carrier gas (helium) flowed at a constant rate of 1.0 mL/min. The desired fatty acid component was analyzed by comparing the retention time to those of the standards.
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