Functional anti mouse cd3e and cd28 antibodies mabs

C57BL/6 and Balb/c mice were purchased from the Jackson Laboratory. Dectin-1 -/-mice were provided by G. Brown (University of Aberdeen, Aberdeen, Scotland). Mice were bred and maintained in pathogen-free facilities at the First Hospital Animal Center of Jilin University. 6 to 8-week-old mice were used for the experiments. All animal experimental procedures were reviewed and approved by the Animal Ethical Committee of First Hospital of Jilin University.
RPMI-1640 medium, penicillin and streptomycin were purchased from Invitrogen. Fetal bovine serum was purchased from Hyclone. Recombinant murine granulocytemacrophage colony-stimulating factor (GM-CSF), IL-4, IL-1β and tumor necrosis factor-alpha (TNF-α) were purchased from Peprotech. Recombinant human transforming growth factor-beta (TGF-β) was purchased from R&D Systems. Functional anti-mouse CD3e and CD28 antibodies (mAbs) were purchased from eBioscience. Curdlan was purchased from Sigma-Aldrich. Lipopolysaccharide (LPS) and poly I:C were purchased from Sigma-Aldrich and Invivogen respectively. Syk inhibitor Piceatannol and Raf-1 kinase inhibitor GW5074 were purchased from Calbiochem. NF-κB inhibitor Bortezomib was purchased from Selleckchem. NF-κB inhibitor (CAS 213546-53-3) was purchased from Santa Cruz, and JSH-23 (a NF-κB inhibitor) was purchased from MCE.