Cells were lysed in extraction buffer (0.1 M Tris-HCl, pH 7.2, 1% TritonX-100, 200 mM NaCl, protease inhibitor cocktail) at 4°C for 30 min. The lysates were subjected to centrifugation at 13,000 rpm for 20 min, and the supernatant was obtained. Blots were incubated with antibodies against anti-TGase1 (Santa Cruz Biotechnology, CA, USA) and β-actin (Santa Cruz Biotechnology). After incubation, membranes were rinsed three times with TBS-T and were incubated with donkey antirabbit IgG antibody (Bethyl Laboratories, Montgomery, TX, USA) and goat anti-mouse IgG antibody (Bio-Rad, CA, USA) for 1 h at room temperature. Binding antibodies were detected using a WEST-ZOL® Plus Western Blot Detection System (INtRON Biotechnology, Sungnam, Korea) and visualized with ChemiDoc XRS (Bio-Rad, Hercules, CA, USA).
Anti tgase1
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-TGase1 is a laboratory product that detects the presence of Transglutaminase 1 (TGase1), a protein involved in the formation of the skin's protective barrier. This product can be used in research and investigative applications that require the identification and quantification of TGase1 levels.
Automatically generated - may contain errors
Lab products found in correlation
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West zol plus western blot detection system, by iNtRON Biotechnology (1 mentions)
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Anti gadph, by Merck Group (1 mentions)
Anti phospho stat3 tyr705, by Cell Signaling Technology (1 mentions)
Anti stat3 c 20, by Santa Cruz Biotechnology (1 mentions)
Hybond p pvdf membrane, by GE Healthcare (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Anti loricrin, by Fortrea (1 mentions)
2 protocols using anti tgase1
1
Protein Extraction and Western Blot Analysis
2
Western Blot Analysis of Epidermal Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protein lysates form cell cultures or skin biopsies were resolved on SDS polyacrylamide gels and blotted onto a Hybond P PVDF membrane (G&E Healthcare, Waukesha, WI, USA).
The following antibodies were used: anti-β-actin (Sigma; 1 : 5000), anti-GADPH (Sigma Aldrich; 1 : 5000), anti-K10 (Covante Inc., Princeton, NJ, USA; 1 : 1000), anti-TGase1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA; 1 : 300), anti-loricrin (Covance; 1 : 1000), anti-p63 (Y4A3 p3362; Sigma-Aldrich; 1 : 1000), anti-phospho-STAT3 (Tyr 705) (Cell Signaling, Danvers, MA, USA; 1 : 1000) anti-Stat3 (C-20) (Santa Cruz; 1 : 100), and anti-SIRT1 (ab13749) (Abcam; 1 : 200).
The following antibodies were used: anti-β-actin (Sigma; 1 : 5000), anti-GADPH (Sigma Aldrich; 1 : 5000), anti-K10 (Covante Inc., Princeton, NJ, USA; 1 : 1000), anti-TGase1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA; 1 : 300), anti-loricrin (Covance; 1 : 1000), anti-p63 (Y4A3 p3362; Sigma-Aldrich; 1 : 1000), anti-phospho-STAT3 (Tyr 705) (Cell Signaling, Danvers, MA, USA; 1 : 1000) anti-Stat3 (C-20) (Santa Cruz; 1 : 100), and anti-SIRT1 (ab13749) (Abcam; 1 : 200).
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