Anti mouse sn clone ser 4

All studies were approved by the UCSD Committee on the Use and Care of Animals and performed using accepted veterinary standards. Generation of Sn-deficient mice has previously been described [19 (link)]. GBS DK23 was labeled with 5-(and-6)-carboxyfluorescein (Invitrogen) per manufacturer’s instructions. Mice were intravenously infected with 2 × 10⁸ CFU of labeled GBS via tail vein. Kidney, lung, and spleen were collected for CFU enumeration or frozen in OCT solution 1 h after infection. Spleen cryostat sections (5 μm) were fixed in acetone and blocked with 1 % BSA/PBST and AVIDIN/BIOTIN blocking kit (Vector Laboratories). The sections were stained with rat mAb anti-mouse Sn (clone SER-4) with Alexa Fluor 647-conjugated goat anti-rat IgG (Life Technologies) followed by second-step staining with biotinylated rat mAb anti-mouse F4/80 (clone CI:A3-1, Serotec) or B220 (clone RA3-6B2, BD Biosciences) with Alexa Fluor 555-conjugated streptavidin (Life Technologies). Images were from an Olympus FV1000 confocal microscope using FV1000 Viewer software.